Anti-cancer activity of quercetin in neuroblastoma: an in vitro approach

Neuroblastoma is a neuroendocrine tumour derived from neural crest cells and it remains a major therapeutic challenge in pediatric oncology. As response rates to chemotherapy are low, surgery remains the only effective treatment but since many tumors have metastasized at the time of diagnosis, curat...

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Bibliographic Details
Published in:Neurological sciences 2014-02, Vol.35 (2), p.163-170
Main Authors: Sugantha Priya, E., Selvakumar, K., Bavithra, S., Elumalai, P., Arunkumar, R., Raja Singh, P., Brindha Mercy, A., Arunakaran, J.
Format: Article
Language:English
Subjects:
Animals
Antineoplastic Agents - pharmacology
Apoptosis - drug effects
Apoptosis - physiology
bcl-2-Associated X Protein - metabolism
Blotting, Western
Caspase 3 - metabolism
Caspase 9 - metabolism
Cell Line, Tumor
Cell Survival - drug effects
Cell Survival - physiology
Cytochromes c - metabolism
Gene Expression - drug effects
Indoles
Medicine
Medicine & Public Health
Mice
Neuroblastoma - drug therapy
Neuroblastoma - physiopathology
Neurology
Neuroradiology
Neurosciences
Neurosurgery
Original Article
Polymerase Chain Reaction
Proteins - metabolism
Psychiatry
Quercetin - pharmacology
RNA, Messenger - metabolism
Tumor Suppressor Protein p53 - metabolism
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Summary:Neuroblastoma is a neuroendocrine tumour derived from neural crest cells and it remains a major therapeutic challenge in pediatric oncology. As response rates to chemotherapy are low, surgery remains the only effective treatment but since many tumors have metastasized at the time of diagnosis, curative surgery is rarely achieved. Consequently, a substantial need for new therapeutic options emerges. Quercetin a flavonoid, has been reported to lower the risk of several cancers. This study was designed to investigate its effects on apoptosis induction in the N2a, a mouse neuroblastoma cell line. The cell viability was determined by dimethyl thiazolyl tetrazolium bromide assay and diamidino-2-phenylindole staining was performed to confirm the apoptosis. The gene expression of bcl-w, p53, p27 and protein expression of caspases (3 and 9), bax, cytochrome-c were studied. This in vitro outcome suggests that quercetin can be used as a potent anti-cancer drug in future.
ISSN:1590-1874
1590-3478
DOI:10.1007/s10072-013-1462-1