Submicron-grooved culture surface extends myotube length by forming parallel and elongated motif

During skeletal muscle development, correct cellular orientation is vital to generate desired longitudinal contraction for functional muscle fibres. In this reported study, submicron-imprint lithography was used to generate submicron-grooved surfaces on polystyrene plates to induce striated myotubes...

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Bibliographic Details
Published in:Micro & nano letters 2013-08, Vol.8 (8), p.440-444
Main Authors: Yuan, Chen-Ching, Ma, Kuang-Jen, Li, Kuei-Chi, Chien, Hsi-Hsin, Lu, Huai-En, Tseng, Ching-Ping, Hwang, Shiaw-Min
Format: Article
Language:English
Subjects:
biomechanics
biomedical materials
cell growth
cell maturation
cell migration
Cellular
cellular orientation
cellular transport
co‐culture submicron‐groove‐grown myotubes
elongated motif
Elongation
Flat surfaces
functional muscle fibres
in‐vitro striated myotubes
Lithography
mouse muscle myoblast cells
muscle
muscle tissue engineering
Muscles
myotube formation
myotube length
Nanostructure
neurophysiology
neurotransmitter secreting cells
Neurotransmitters
parallel motif
parallel myotubes
physiological functionality
polymers
polystyrene plates
Polystyrene resins
skeletal muscle development
striated motifs
submicron‐grooved culture surface
submicron‐imprint lithography
tissue engineering
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Summary:During skeletal muscle development, correct cellular orientation is vital to generate desired longitudinal contraction for functional muscle fibres. In this reported study, submicron-imprint lithography was used to generate submicron-grooved surfaces on polystyrene plates to induce striated myotubes in vitro. Mouse muscle myoblast cells cultured on a submicron-grooved surface migrated faster in a directionally uniform fashion; in comparison, cells cultured on a flat surface grew and migrated slower in indiscriminate directions. Subsequent maturation of the myoblast cells formed along the submicron-groove surface resulted in a tandem of parallel myotubes that were both longer and greater in circumference than in the case of the flat surface. In a functional test, the co-culture submicron-groove-grown myotubes with neurotransmitter secreting cells further demonstrated contraction abilities, suggesting submicron-groove-guided growth served to enhance myotube formation while retaining striated motifs and physiological functionality for muscle tissue engineering.
ISSN:1750-0443
1750-0443
DOI:10.1049/mnl.2013.0153