Leaf physiology and morphology of Castanea dentata (Marsh.) Borkh., Castanea mollissima Blume, and three backcross breeding generations planted in the southern Appalachians, USA

• Published in: New forests 2014-03, Vol.45 (2), p.283-293
• Main Authors: Knapp, Benjamin O, Wang, G. Geoff, Clark, Stacy L, Pile, Lauren S, Schlarbaum, Scott E
• Format: Article
• Language: English
• Subjects: backcrossing; Biomedical and Life Sciences; Castanea dentata; Castanea mollissima; chestnuts; Cryphonectria parasitica; Disease control; Disease resistance; Forest management; forest trees; Forestry; fungi; Growing season; hybrids; Indigenous species; leaf area; Leaves; Life Sciences; Marshes; Pathogens; Photosynthesis; Physiology; Plant propagation; Plant species; planting; Seedlings; Selective breeding; Short Communication; Studies; Threatened species
• ISSN: 0169-4286; 1573-5095
• DOI: 10.1007/s11056-014-9408-x

Backcross breeding programs have been used to transfer disease resistance and other traits from one forest tree species to another in order to meet restoration objectives. Evaluating the field performance of such material is critical for determining the success of breeding programs. In eastern North America, The American Chestnut Foundation has a backcross breeding program that uses Chinese chestnut (Castanea mollissima Blume) to introduce resistance of the fungal pathogen chestnut blight [Cryphonectria parasitica (Murr.) Barr.] to the native American chestnut [Castanea dentata (Marsh.) Borkh.]. We compared physiological and morphological characteristics among seedlings of American chestnut, Chinese chestnut, and BC₁F₃, BC₂F₃, and BC₃F₃ hybrid chestnuts during their fourth growing season after field-planting. American chestnut and the BC₃F₃ breeding generation displayed photosynthetic light-response curves that were similar to each other but different from Chinese chestnut. Rates of photosynthesis were higher for American chestnut and the BC₃F₃ breeding generation when compared to Chinese chestnut for light levels ≥800 μmol m⁻² s⁻¹ photosynthetic photon flux density and for maximum photosynthetic capacity. Leaf morphology variables were not different between American chestnut and any of the breeding generations, but leaf area (on a per leaf basis) of Chinese chestnut was lower than that of any other chestnut type. Our results suggest that backcross breeding can be used to transfer desirable traits for restoration of native species threatened by non-native pathogens.