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KCC2 Interacts with the Dendritic Cytoskeleton to Promote Spine Development
The neuron-specific K-Cl cotransporter, KCC2, induces a developmental shift to render GABAergic transmission from depolarizing to hyperpolarizing. Now we demonstrate that KCC2, independently of its Cl − transport function, is a key factor in the maturation of dendritic spines. This morphogenic role...
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| Published in: | Neuron (Cambridge, Mass.) Mass.), 2007-12, Vol.56 (6), p.1019-1033 |
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| Main Authors: | , , , , , , , , , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c434t-6c8e4185a5480fc1ea6ea6b8c0090b464c3ba917a62efb17a764b186071e3eb83 |
| container_end_page | 1033 |
| container_issue | 6 |
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| container_title | Neuron (Cambridge, Mass.) |
| container_volume | 56 |
| creator | Li, Hong Khirug, Stanislav Cai, Chunlin Ludwig, Anastasia Blaesse, Peter Kolikova, Julia Afzalov, Ramil Coleman, Sarah K. Lauri, Sari Airaksinen, Matti S. Keinänen, Kari Khiroug, Leonard Saarma, Mart Kaila, Kai Rivera, Claudio |
| description | The neuron-specific K-Cl cotransporter, KCC2, induces a developmental shift to render GABAergic transmission from depolarizing to hyperpolarizing. Now we demonstrate that KCC2, independently of its Cl
− transport function, is a key factor in the maturation of dendritic spines. This morphogenic role of KCC2 in the development of excitatory synapses is mediated by structural interactions between KCC2 and the spine cytoskeleton. Here, the binding of KCC2 C-terminal domain to the cytoskeleton-associated protein 4.1N may play an important role. A more general conclusion based on our data is that KCC2 acts as a synchronizing factor in the functional development of glutamatergic and GABAergic synapses in cortical neurons and networks. |
| doi_str_mv | 10.1016/j.neuron.2007.10.039 |
| format | article |
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A more general conclusion based on our data is that KCC2 acts as a synchronizing factor in the functional development of glutamatergic and GABAergic synapses in cortical neurons and networks.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>CELLBIO</subject><subject>Cells, Cultured</subject><subject>Cerebral Cortex - cytology</subject><subject>Cerebral Cortex - embryology</subject><subject>Cerebral Cortex - growth & development</subject><subject>Cytoskeletal Proteins</subject><subject>Cytoskeleton</subject><subject>Cytoskeleton - physiology</subject><subject>Dendrites - metabolism</subject><subject>Dendrites - ultrastructure</subject><subject>Dendritic Spines - physiology</subject><subject>Embryo, Mammalian</subject><subject>Excitatory Postsynaptic Potentials - drug effects</subject><subject>Excitatory Postsynaptic Potentials - physiology</subject><subject>Excitatory Postsynaptic Potentials - radiation effects</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Humans</subject><subject>Immunization</subject><subject>In Vitro Techniques</subject><subject>K Cl- Cotransporters</subject><subject>Lysine - analogs & derivatives</subject><subject>Lysine - metabolism</subject><subject>Membrane Proteins</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Microscopy</subject><subject>Mutation - physiology</subject><subject>Nerve Tissue Proteins</subject><subject>Neurons</subject><subject>Neurons - cytology</subject><subject>Neuropeptides</subject><subject>Patch-Clamp Techniques - methods</subject><subject>Proteins</subject><subject>Symporters - deficiency</subject><subject>Symporters - physiology</subject><subject>Synaptic Transmission - physiology</subject><subject>SYSBIO</subject><subject>SYSNEURO</subject><subject>Transfection - methods</subject><issn>0896-6273</issn><issn>1097-4199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNp9kNtKAzEQhoMoWg9vILLg9dbJJptNbgRZjygoqNdhN53S1DapSVrp25vSgnfCwA_DNzPMR8g5hSEFKq6mQ4fL4N2wAmhyawhM7ZEBBdWUnCq1TwYglShF1bAjchzjFIDyWtFDckQlKFZXfECen9u2Kp5cwtCZFIsfmyZFmmBxi24UbLKmaNfJxy-cYfKuSL54C37uExbvC-s23ApnfjFHl07JwbibRTzb5Qn5vL_7aB_Ll9eHp_bmpTSc8VQKI5FTWXc1lzA2FDuRq5cGQEHPBTes7xRtOlHhuM_ZCN5TKaChyLCX7IRcbvcugv9eYkx66pfB5ZOa1sCEkIo1meJbygQfY8CxXgQ778JaU9Abg3qqtwb1xuCmmw3msYvd8mU_x9Hf0E5ZBq63AOYXVxaDjsaiMziyAU3SI2__v_AL42uDNQ</recordid><startdate>20071220</startdate><enddate>20071220</enddate><creator>Li, Hong</creator><creator>Khirug, Stanislav</creator><creator>Cai, Chunlin</creator><creator>Ludwig, Anastasia</creator><creator>Blaesse, Peter</creator><creator>Kolikova, Julia</creator><creator>Afzalov, Ramil</creator><creator>Coleman, Sarah K.</creator><creator>Lauri, Sari</creator><creator>Airaksinen, Matti S.</creator><creator>Keinänen, Kari</creator><creator>Khiroug, Leonard</creator><creator>Saarma, Mart</creator><creator>Kaila, Kai</creator><creator>Rivera, Claudio</creator><general>Elsevier Inc</general><general>Elsevier Limited</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20071220</creationdate><title>KCC2 Interacts with the Dendritic Cytoskeleton to Promote Spine Development</title><author>Li, Hong ; Khirug, Stanislav ; Cai, Chunlin ; Ludwig, Anastasia ; Blaesse, Peter ; Kolikova, Julia ; Afzalov, Ramil ; Coleman, Sarah K. ; Lauri, Sari ; Airaksinen, Matti S. ; Keinänen, Kari ; Khiroug, Leonard ; Saarma, Mart ; Kaila, Kai ; Rivera, Claudio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-6c8e4185a5480fc1ea6ea6b8c0090b464c3ba917a62efb17a764b186071e3eb83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>CELLBIO</topic><topic>Cells, Cultured</topic><topic>Cerebral Cortex - cytology</topic><topic>Cerebral Cortex - embryology</topic><topic>Cerebral Cortex - growth & development</topic><topic>Cytoskeletal Proteins</topic><topic>Cytoskeleton</topic><topic>Cytoskeleton - physiology</topic><topic>Dendrites - metabolism</topic><topic>Dendrites - ultrastructure</topic><topic>Dendritic Spines - physiology</topic><topic>Embryo, Mammalian</topic><topic>Excitatory Postsynaptic Potentials - drug effects</topic><topic>Excitatory Postsynaptic Potentials - physiology</topic><topic>Excitatory Postsynaptic Potentials - radiation effects</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Humans</topic><topic>Immunization</topic><topic>In Vitro Techniques</topic><topic>K Cl- Cotransporters</topic><topic>Lysine - analogs & derivatives</topic><topic>Lysine - metabolism</topic><topic>Membrane Proteins</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Microscopy</topic><topic>Mutation - physiology</topic><topic>Nerve Tissue Proteins</topic><topic>Neurons</topic><topic>Neurons - cytology</topic><topic>Neuropeptides</topic><topic>Patch-Clamp Techniques - methods</topic><topic>Proteins</topic><topic>Symporters - deficiency</topic><topic>Symporters - physiology</topic><topic>Synaptic Transmission - physiology</topic><topic>SYSBIO</topic><topic>SYSNEURO</topic><topic>Transfection - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Hong</creatorcontrib><creatorcontrib>Khirug, Stanislav</creatorcontrib><creatorcontrib>Cai, Chunlin</creatorcontrib><creatorcontrib>Ludwig, Anastasia</creatorcontrib><creatorcontrib>Blaesse, Peter</creatorcontrib><creatorcontrib>Kolikova, Julia</creatorcontrib><creatorcontrib>Afzalov, Ramil</creatorcontrib><creatorcontrib>Coleman, Sarah K.</creatorcontrib><creatorcontrib>Lauri, Sari</creatorcontrib><creatorcontrib>Airaksinen, Matti S.</creatorcontrib><creatorcontrib>Keinänen, Kari</creatorcontrib><creatorcontrib>Khiroug, Leonard</creatorcontrib><creatorcontrib>Saarma, Mart</creatorcontrib><creatorcontrib>Kaila, Kai</creatorcontrib><creatorcontrib>Rivera, Claudio</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Neuron (Cambridge, Mass.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Hong</au><au>Khirug, Stanislav</au><au>Cai, Chunlin</au><au>Ludwig, Anastasia</au><au>Blaesse, Peter</au><au>Kolikova, Julia</au><au>Afzalov, Ramil</au><au>Coleman, Sarah K.</au><au>Lauri, Sari</au><au>Airaksinen, Matti S.</au><au>Keinänen, Kari</au><au>Khiroug, Leonard</au><au>Saarma, Mart</au><au>Kaila, Kai</au><au>Rivera, Claudio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>KCC2 Interacts with the Dendritic Cytoskeleton to Promote Spine Development</atitle><jtitle>Neuron (Cambridge, Mass.)</jtitle><addtitle>Neuron</addtitle><date>2007-12-20</date><risdate>2007</risdate><volume>56</volume><issue>6</issue><spage>1019</spage><epage>1033</epage><pages>1019-1033</pages><issn>0896-6273</issn><eissn>1097-4199</eissn><abstract>The neuron-specific K-Cl cotransporter, KCC2, induces a developmental shift to render GABAergic transmission from depolarizing to hyperpolarizing. Now we demonstrate that KCC2, independently of its Cl
− transport function, is a key factor in the maturation of dendritic spines. This morphogenic role of KCC2 in the development of excitatory synapses is mediated by structural interactions between KCC2 and the spine cytoskeleton. Here, the binding of KCC2 C-terminal domain to the cytoskeleton-associated protein 4.1N may play an important role. A more general conclusion based on our data is that KCC2 acts as a synchronizing factor in the functional development of glutamatergic and GABAergic synapses in cortical neurons and networks.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>18093524</pmid><doi>10.1016/j.neuron.2007.10.039</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Neuron (Cambridge, Mass.), 2007-12, Vol.56 (6), p.1019-1033 |
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| source | BACON - Elsevier - GLOBAL_SCIENCEDIRECT-OPENACCESS |
| subjects | Animals Animals, Newborn CELLBIO Cells, Cultured Cerebral Cortex - cytology Cerebral Cortex - embryology Cerebral Cortex - growth & development Cytoskeletal Proteins Cytoskeleton Cytoskeleton - physiology Dendrites - metabolism Dendrites - ultrastructure Dendritic Spines - physiology Embryo, Mammalian Excitatory Postsynaptic Potentials - drug effects Excitatory Postsynaptic Potentials - physiology Excitatory Postsynaptic Potentials - radiation effects Green Fluorescent Proteins - metabolism Humans Immunization In Vitro Techniques K Cl- Cotransporters Lysine - analogs & derivatives Lysine - metabolism Membrane Proteins Mice Mice, Knockout Microscopy Mutation - physiology Nerve Tissue Proteins Neurons Neurons - cytology Neuropeptides Patch-Clamp Techniques - methods Proteins Symporters - deficiency Symporters - physiology Synaptic Transmission - physiology SYSBIO SYSNEURO Transfection - methods |
| title | KCC2 Interacts with the Dendritic Cytoskeleton to Promote Spine Development |
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