High-throughput fluorescent multiplex array for indoor allergen exposure assessment

Background Current enzyme immunoassay methods for detection of common indoor allergens in environmental dust samples are labor-intensive and time consuming. Objective To develop and validate a fluorescent multiplex array to measure 6 (Der p 1, Der f 1, Der p 2, Der f 2, Fel d 1, and Can f 1) indoor...

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Bibliographic Details
Published in:Journal of allergy and clinical immunology 2007-02, Vol.119 (2), p.428-433
Main Authors: Earle, Christopher D., MA, King, Eva M., PhD, Tsay, Amy, MS, Pittman, Kimberly, BS, Saric, Branka, MA, Vailes, Lisa, MS, Godbout, Rebecca, BS, Oliver, Kerry G., PhD, Chapman, Martin D., PhD
Format: Article
Language:English
Subjects:
Air Pollution, Indoor
Allergens - analysis
Allergies
Allergy and Immunology
asthma
Biological and medical sciences
Dust
environment
Environmental Monitoring - methods
Enzyme-Linked Immunosorbent Assay
exposure
Fluorescence
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Immunoassay
Immunopathology
indoor air quality
Indoor allergen
Medical sciences
multiplex array
Outdoor air quality
Proteins
Reproducibility of Results
Sensitivity and Specificity
Studies
Technological change
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Summary:Background Current enzyme immunoassay methods for detection of common indoor allergens in environmental dust samples are labor-intensive and time consuming. Objective To develop and validate a fluorescent multiplex array to measure 6 (Der p 1, Der f 1, Der p 2, Der f 2, Fel d 1, and Can f 1) indoor allergen levels simultaneously. Methods A multiplex array for 6 allergens, using mAbs covalently coupled to fluorescent microspheres, was developed using a single universal standard composed of purified natural allergens. The multiplex array was validated by comparing the measured dust mite, cat, and dog allergen levels in household dust samples to those obtained by standard ELISA methods. Results Linear regression analysis showed a highly significant quantitative correlation between the multiplex array and ELISA for dust mite, cat, and dog allergens: R2 values ranging from 0.90 to 0.99 ( P < .001). In addition, the sensitivity, limit of detection (
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2006.11.004