Effect of cadmium on cytogenetic toxicity in hairy roots of Wedelia trilobata L. and their alleviation by exogenous CaCl^sub 2

Effects of cadmium (Cd) alone and in combination with calcium on mitosis and chromosomal aberration in the hairy root tips of Wedelia trilobata were investigated. The results showed that Cd concentrations below 50 [mu]mol/L had a lesser or even a promoting effect on the mitotic index (MI) and the ra...

Full description

Saved in:
Bibliographic Details
Published in:Environmental science and pollution research international 2014-01, Vol.21 (2), p.1436
Main Authors: Shi, He Ping, Zhu, Yuan Feng, Wang, Yun Ling, Tsang, Po Keung, Eric
Format: Article
Language:English
Subjects:
Cadmium
Calcium
Cell division
Chromosome aberrations
Chromosomes
Cytogenetics
Cytotoxicity
Environmental monitoring
Hairy root
Heavy metal content
Heavy metals
Investigations
Micronuclei
Mitosis
Mutagenesis
Phytotoxicity
Plant growth
Pollutants
Roots
Sensitivity analysis
Soil contamination
Studies
Toxicity
Wedelia trilobata
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Effects of cadmium (Cd) alone and in combination with calcium on mitosis and chromosomal aberration in the hairy root tips of Wedelia trilobata were investigated. The results showed that Cd concentrations below 50 [mu]mol/L had a lesser or even a promoting effect on the mitotic index (MI) and the rate of chromosomal aberration in hairy root tips, while those higher than 100 [mu]mol/L significantly decreased the MI and gradually stimulated the rate of chromosomal aberrations with prolonged time and increasing concentrations of Cd. Concentrations of 50 [mu]mol/L Cd mainly induced C-mitosis, while more than 100 [mu]mol/L Cd mainly caused chromosome breakage and chromosome adhesion in hairy root tip cells. When cultured with 300 [mu]mol/L Cd, micronuclei were only observed in the interphase, middle, and late phase of hairy root tip cells. Compared with untreated controls, exogenous calcium had an alleviating effect on Cd-induced cytotoxicity by effectively enhancing the MI and reducing the rate of chromosomal aberration in root tip cells. The results presented here provide evidence that W. trilobata hairy roots with rapid autonomous growth could be used as a sensitive tool for monitoring and evaluation of Cd pollution in the environment.[PUBLICATION ABSTRACT]
ISSN:0944-1344
1614-7499
DOI:10.1007/s11356-013-2015-0