The Abundance of Lipid Transfer Protein (LTP), the Major Food Allergen of Corn, Varies Between Hybrids and Growth Conditions

Extracts were adjusted to equal protein concentrations, mixed with reducing Laemmli buffer, heated, and then 2 μL samples were spotted on nitrocellulose membranes before incubating with LTP-specific rabbit IgG.

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Bibliographic Details
Published in:Journal of allergy and clinical immunology 2009-02, Vol.123 (2), p.S28-S28
Main Authors: Ariyarathna, H, Pramod, S.N, Goodman, R.E
Format: Article
Language:English
Subjects:
Allergy and Immunology
Proteins
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Description
Summary:Extracts were adjusted to equal protein concentrations, mixed with reducing Laemmli buffer, heated, and then 2 μL samples were spotted on nitrocellulose membranes before incubating with LTP-specific rabbit IgG.
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2008.12.121