Basophil Activation Induces Rapid Membrane Reorganization, Polarization and the Formation of Putative Intercellular Nanotubes

Human basophils were enriched from buffy coats by magnetic beads. fMLP (1 uM) and polyclonal anti-IgE (0.5 ug/ml) were used to stimulate the cells for multiple time points.

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Bibliographic Details
Published in:Journal of allergy and clinical immunology 2009-02, Vol.123 (2), p.S198-S198
Main Authors: Gross, M.G, Masilimani, M, Sampson, H.A, Shreffler, W.G
Format: Article
Language:English
Subjects:
Allergy and Immunology
Flow cytometry
Microscopy
Online Access:Get full text
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Description
Summary:Human basophils were enriched from buffy coats by magnetic beads. fMLP (1 uM) and polyclonal anti-IgE (0.5 ug/ml) were used to stimulate the cells for multiple time points.
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2008.12.755