Time course of allergen induced nitric oxide synthase mRNA expression in mice model of anaphylaxis

The inducible isoform of nitric oxide synthase (iNOS) was implicated in the pathogenesis of anaphylaxis. iNOS activation depends on de novo synthesis of both RNA and protein. Therefore it is assumed that several hours are required for its full activation. Yet the hemodynamic response in anaphylaxis...

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Bibliographic Details
Published in:Journal of allergy and clinical immunology 2004-02, Vol.113 (2), p.S249-S249
Main Authors: Sade, K., Etkin, S., Kivity, S.
Format: Article
Language:English
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Summary:The inducible isoform of nitric oxide synthase (iNOS) was implicated in the pathogenesis of anaphylaxis. iNOS activation depends on de novo synthesis of both RNA and protein. Therefore it is assumed that several hours are required for its full activation. Yet the hemodynamic response in anaphylaxis has been documented as early as minutes after allergen exposure. Experiments were designed to determine the time course of allergen induced iNOS mRNA expression and activity in mice model of anaphylaxis. BALB/c mice were actively sensitized and challenged by ovalbumin to induce anaphylaxis. Tissues were removed and blood was drawn in different time points during 16 hours after the induction of anaphylaxis. Nitric oxide generation was measured with a Griess assay, and lung and heart iNOS mRNA was studied by reverse transcriptase-polymerase chain reaction. A significant increase in lung and heart iNOS mRNA expression was evident as early as 10 minutes after allergen challenge. The quantity of iNOS mRNA reached its peak (p
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2004.01.360