Intracellular cytokine production by T cells in SCID infants with time post-bone marrow transplantation (BMT)

To assess whether there is immune deviation in T cells of SCIDs with time post-BMT, intracellular cytokine production by T cells was studied. PBMC were incubated with PMA/Ionomycin and 4-color flow cytometry was performed to detect intracytoplasmic IL2, IL4 and IFNγ and surface CD3, CD8, CD45RA and...

Full description

Saved in:
Bibliographic Details
Published in:Journal of allergy and clinical immunology 2004-02, Vol.113 (2), p.S42-S42
Main Authors: Sajaroff, E.O., Buckley, R.H.
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To assess whether there is immune deviation in T cells of SCIDs with time post-BMT, intracellular cytokine production by T cells was studied. PBMC were incubated with PMA/Ionomycin and 4-color flow cytometry was performed to detect intracytoplasmic IL2, IL4 and IFNγ and surface CD3, CD8, CD45RA and CD45RO simultaneously. Analyses were done sequentially post-BMT in 5 infants (4 SCID-X1 and 1 Jak3-deficient). Six long-term survivors were studied once (2 IL7Rα deficient and 4 SCID-Xl). The percentage of CD3+ T cells positive for IL-2 was lower than in infant and adult controls (41.5+/-7.8%), in 4 of 5 infants early after transplantation ( p
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2003.12.109