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Intracellular cytokine production by T cells in SCID infants with time post-bone marrow transplantation (BMT)
To assess whether there is immune deviation in T cells of SCIDs with time post-BMT, intracellular cytokine production by T cells was studied. PBMC were incubated with PMA/Ionomycin and 4-color flow cytometry was performed to detect intracytoplasmic IL2, IL4 and IFNγ and surface CD3, CD8, CD45RA and...
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| Published in: | Journal of allergy and clinical immunology 2004-02, Vol.113 (2), p.S42-S42 |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
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| container_end_page | S42 |
| container_issue | 2 |
| container_start_page | S42 |
| container_title | Journal of allergy and clinical immunology |
| container_volume | 113 |
| creator | Sajaroff, E.O. Buckley, R.H. |
| description | To assess whether there is immune deviation in T cells of SCIDs with time post-BMT, intracellular cytokine production by T cells was studied.
PBMC were incubated with PMA/Ionomycin and 4-color flow cytometry was performed to detect intracytoplasmic IL2, IL4 and IFNγ and surface CD3, CD8, CD45RA and CD45RO simultaneously. Analyses were done sequentially post-BMT in 5 infants (4 SCID-X1 and 1 Jak3-deficient). Six long-term survivors were studied once (2 IL7Rα deficient and 4 SCID-Xl).
The percentage of CD3+ T cells positive for IL-2 was lower than in infant and adult controls (41.5+/-7.8%), in 4 of 5 infants early after transplantation (
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| doi_str_mv | 10.1016/j.jaci.2003.12.109 |
| format | article |
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PBMC were incubated with PMA/Ionomycin and 4-color flow cytometry was performed to detect intracytoplasmic IL2, IL4 and IFNγ and surface CD3, CD8, CD45RA and CD45RO simultaneously. Analyses were done sequentially post-BMT in 5 infants (4 SCID-X1 and 1 Jak3-deficient). Six long-term survivors were studied once (2 IL7Rα deficient and 4 SCID-Xl).
The percentage of CD3+ T cells positive for IL-2 was lower than in infant and adult controls (41.5+/-7.8%), in 4 of 5 infants early after transplantation (
p<0.05) but normalized at between 60 and 114 days post-BMT. In contrast, 4 of 5 infants had higher percentages of T cells positive for IFNγ and IL-4 early after BMT than did age-matched controls who had <1% for both (
p<0.05). Four of 5 infants normalized IFNγ and IL-4 production between 120 and 140 days and 60 and 114 days post BMT, respectively. The long-term survivors had a normal percentage of IL-4 producing T cells (1.5+/-0.9% of CD3+cells), and 3 of 5 had percentages of IFNγ positive T cells within the adult normal range (26.9+/-7.3%). Although 4 of 6 had lower than normal percentages of IL-2 producing T cells, T cell responses to mitogens and antigens were normal in 5 of the 6.
T cells in SCID infants early after BMT produce different cytokines than do those later, and the changes correlate temporally with the development of immunocompetence.</description><identifier>ISSN: 0091-6749</identifier><identifier>EISSN: 1097-6825</identifier><identifier>DOI: 10.1016/j.jaci.2003.12.109</identifier><language>eng</language><publisher>St. Louis: Mosby, Inc</publisher><ispartof>Journal of allergy and clinical immunology, 2004-02, Vol.113 (2), p.S42-S42</ispartof><rights>2004</rights><rights>Copyright Elsevier Limited Feb 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Sajaroff, E.O.</creatorcontrib><creatorcontrib>Buckley, R.H.</creatorcontrib><title>Intracellular cytokine production by T cells in SCID infants with time post-bone marrow transplantation (BMT)</title><title>Journal of allergy and clinical immunology</title><description>To assess whether there is immune deviation in T cells of SCIDs with time post-BMT, intracellular cytokine production by T cells was studied.
PBMC were incubated with PMA/Ionomycin and 4-color flow cytometry was performed to detect intracytoplasmic IL2, IL4 and IFNγ and surface CD3, CD8, CD45RA and CD45RO simultaneously. Analyses were done sequentially post-BMT in 5 infants (4 SCID-X1 and 1 Jak3-deficient). Six long-term survivors were studied once (2 IL7Rα deficient and 4 SCID-Xl).
The percentage of CD3+ T cells positive for IL-2 was lower than in infant and adult controls (41.5+/-7.8%), in 4 of 5 infants early after transplantation (
p<0.05) but normalized at between 60 and 114 days post-BMT. In contrast, 4 of 5 infants had higher percentages of T cells positive for IFNγ and IL-4 early after BMT than did age-matched controls who had <1% for both (
p<0.05). Four of 5 infants normalized IFNγ and IL-4 production between 120 and 140 days and 60 and 114 days post BMT, respectively. The long-term survivors had a normal percentage of IL-4 producing T cells (1.5+/-0.9% of CD3+cells), and 3 of 5 had percentages of IFNγ positive T cells within the adult normal range (26.9+/-7.3%). Although 4 of 6 had lower than normal percentages of IL-2 producing T cells, T cell responses to mitogens and antigens were normal in 5 of the 6.
T cells in SCID infants early after BMT produce different cytokines than do those later, and the changes correlate temporally with the development of immunocompetence.</description><issn>0091-6749</issn><issn>1097-6825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNotkE1PwzAMhiMEEmPwBzhF4gKHjiRt01TiAuNr0hAHeo_SxBUpXTuSlGn_npRxsWXr8Wv7ReiSkgUllN-2i1Zpu2CEpAvKYq88QrMYi4QLlh-jGSElTXiRlafozPuWxDoV5QxtVn1wSkPXjZ1yWO_D8GV7wFs3mFEHO_S43uMKT4THtscfy9VjzI3qg8c7Gz5xsJvIDz4k9RAnN8q5YYejau-3XcTUn8r1w1t1c45OGtV5uPjPc1Q9P1XL12T9_rJa3q8TEJQnhjasFlAy3giTFZDlRlMFNa-hYCqvOfA0N4SazNQAolG1TglEsCiE1pylc3R1kI1ffI_gg2yH0fVxo6Q5yUSRMsEjdXegIF7yY8FJry30Gox1oIM0g5WUyMlf2crJXzn5KymLvTL9Bdbfcck</recordid><startdate>20040201</startdate><enddate>20040201</enddate><creator>Sajaroff, E.O.</creator><creator>Buckley, R.H.</creator><general>Mosby, Inc</general><general>Elsevier Limited</general><scope>7SS</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope></search><sort><creationdate>20040201</creationdate><title>Intracellular cytokine production by T cells in SCID infants with time post-bone marrow transplantation (BMT)</title><author>Sajaroff, E.O. ; Buckley, R.H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e816-d1f2b8e926f8d47e45dc1aeb6be72a5b6e635d01d4dbee8fabc30e47e778cc623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sajaroff, E.O.</creatorcontrib><creatorcontrib>Buckley, R.H.</creatorcontrib><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><jtitle>Journal of allergy and clinical immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sajaroff, E.O.</au><au>Buckley, R.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intracellular cytokine production by T cells in SCID infants with time post-bone marrow transplantation (BMT)</atitle><jtitle>Journal of allergy and clinical immunology</jtitle><date>2004-02-01</date><risdate>2004</risdate><volume>113</volume><issue>2</issue><spage>S42</spage><epage>S42</epage><pages>S42-S42</pages><issn>0091-6749</issn><eissn>1097-6825</eissn><abstract>To assess whether there is immune deviation in T cells of SCIDs with time post-BMT, intracellular cytokine production by T cells was studied.
PBMC were incubated with PMA/Ionomycin and 4-color flow cytometry was performed to detect intracytoplasmic IL2, IL4 and IFNγ and surface CD3, CD8, CD45RA and CD45RO simultaneously. Analyses were done sequentially post-BMT in 5 infants (4 SCID-X1 and 1 Jak3-deficient). Six long-term survivors were studied once (2 IL7Rα deficient and 4 SCID-Xl).
The percentage of CD3+ T cells positive for IL-2 was lower than in infant and adult controls (41.5+/-7.8%), in 4 of 5 infants early after transplantation (
p<0.05) but normalized at between 60 and 114 days post-BMT. In contrast, 4 of 5 infants had higher percentages of T cells positive for IFNγ and IL-4 early after BMT than did age-matched controls who had <1% for both (
p<0.05). Four of 5 infants normalized IFNγ and IL-4 production between 120 and 140 days and 60 and 114 days post BMT, respectively. The long-term survivors had a normal percentage of IL-4 producing T cells (1.5+/-0.9% of CD3+cells), and 3 of 5 had percentages of IFNγ positive T cells within the adult normal range (26.9+/-7.3%). Although 4 of 6 had lower than normal percentages of IL-2 producing T cells, T cell responses to mitogens and antigens were normal in 5 of the 6.
T cells in SCID infants early after BMT produce different cytokines than do those later, and the changes correlate temporally with the development of immunocompetence.</abstract><cop>St. Louis</cop><pub>Mosby, Inc</pub><doi>10.1016/j.jaci.2003.12.109</doi></addata></record> |
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| title | Intracellular cytokine production by T cells in SCID infants with time post-bone marrow transplantation (BMT) |
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