Cord Blood Eosinophil Progenitor Expression of Major Basic Protein mRNA is Associated with Maternal Atopic Sensitization

Cells were incubated with 1 ng/mL rhIL-5; at 24, 48 and 72h post-stimulation, RNA was isolated, reverse transcribed, and expression of MBP mRNA determined, utilizing multiplex Q-PCR with GAPDH and beta-actin as housekeeping genes.

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Bibliographic Details
Published in:Journal of allergy and clinical immunology 2010-02, Vol.125 (2), p.AB231-AB231
Main Authors: Ellis, A.K, Fraser, K, Crawford, L, Denburg, J.A
Format: Article
Language:English
Subjects:
Allergy and Immunology
Medical research
Online Access:Get full text
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Description
Summary:Cells were incubated with 1 ng/mL rhIL-5; at 24, 48 and 72h post-stimulation, RNA was isolated, reverse transcribed, and expression of MBP mRNA determined, utilizing multiplex Q-PCR with GAPDH and beta-actin as housekeeping genes.
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2009.12.901