Rosemary-stimulated reduction of DNA strand breaks and FPG-sensitive sites in mammalian cells treated with H2O2 or visible light-excited Methylene Blue

• Published in: Cancer letters 2002-03, Vol.177 (2), p.145-153
• Main Authors: SLAMENOVA, Darina, KUBOSKOVA, Katarina, HORVATHOVA, Eva, ROBICHOVA, Sona
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants; Biological and medical sciences; Caco-2 Cells; Carcinogenesis, carcinogens and anticarcinogens; Carcinogens; Cell metabolism, cell oxidation; Cell physiology; Cell Survival - drug effects; Colorectal cancer; Cricetinae; Deoxyribonucleic acid; Diet; DNA; DNA Damage - drug effects; DNA repair; DNA-Formamidopyrimidine Glycosylase; Enzymes; Food; Foods and miscellaneous; Fundamental and applied biological sciences. Psychology; HIV; Human immunodeficiency virus; Humans; Hydrogen Peroxide - toxicity; Investigations; Light; Lipids; Medical sciences; Metabolites; Methylene Blue; Molecular and cellular biology; N-Glycosyl Hydrolases; Oxidants - toxicity; Plant Preparations - pharmacology; Rodents; Rosmarinus; Skin; Tumorigenesis; Tumors
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/S0304-3835(01)00784-4

In this study possible protective effects of rosemary against oxidative DNA damage induced by H2O2- and visible light-excited Methylene Blue in colon cancer cells CaCo-2 and hamster lung cells V79 were investigated. The level of DNA damage (DNA strand breaks) was measured using the classical and modified single cell gel electrophoresis, so-called comet assay. Our findings showed that an ethanol extract from rosemary reduced the genotoxic activity of both agents after a long-term (24 h; 0.3 microg/ml) or short-term (2 h; 30 microg/ml) pre-incubation of cells. We suggest that the extract of rosemary exhibits a protective effect against oxidative damage to DNA as a consequence of scavenging of both *OH radicals and singlet oxygen ((1)O2).