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Effects of vitamins C and E on cytotoxicity induced by N-nitroso compounds, N-nitrosomorpholine and N-methyl- N′-nitro- N-nitrosoguanidine in Caco-2 and V79 cell lines
Since N-nitroso compounds as strong carcinogens are closely related to food and nutrition, the cytotoxic effects of N-nitrosomorpholine (NMOR) and N-methyl- N′-nitro- N-nitrosoguanidine (MNNG) and their reduction by vitamins C and E were investigated in hamster V79 cells and human colon carcinoma Ca...
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| Published in: | Cancer letters 2002-08, Vol.182 (1), p.11-18 |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c485t-bc9e663c058387bb9d320be6215eeff4809099a6150bda782552c581058a30ef3 |
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| cites | cdi_FETCH-LOGICAL-c485t-bc9e663c058387bb9d320be6215eeff4809099a6150bda782552c581058a30ef3 |
| container_end_page | 18 |
| container_issue | 1 |
| container_start_page | 11 |
| container_title | Cancer letters |
| container_volume | 182 |
| creator | ROBICHOVA, Sona SLAMENOVA, Darina |
| description | Since
N-nitroso compounds as strong carcinogens are closely related to food and nutrition, the cytotoxic effects of
N-nitrosomorpholine (NMOR) and
N-methyl-
N′-nitro-
N-nitrosoguanidine (MNNG) and their reduction by vitamins C and E were investigated in hamster V79 cells and human colon carcinoma Caco-2 cells. Cytotoxicity was evaluated by the trypan blue exclusion technique in Caco-2 cells and by the plating efficiency assay in V79 cells. NMOR caused a dose-dependent decline of viable cells in both cell lines; MNNG induced a dose-dependent cytotoxic effect only in V79 cells. Pretreatment of cells with vitamin C and vitamin E significantly reduced the cytotoxicity of NMOR, however, both vitamins had not effect on cytotoxicity induced by MNNG. These results suggest that different
N-nitroso compounds react differently with cellular macromolecules. Measurement of the level of NMOR-induced DNA strand breaks and alkali-labile sites in both cell types using the alkaline comet assay also indicates a protective effect of both vitamins against the genotoxic effects of NMOR. |
| doi_str_mv | 10.1016/S0304-3835(02)00056-3 |
| format | article |
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N-nitroso compounds as strong carcinogens are closely related to food and nutrition, the cytotoxic effects of
N-nitrosomorpholine (NMOR) and
N-methyl-
N′-nitro-
N-nitrosoguanidine (MNNG) and their reduction by vitamins C and E were investigated in hamster V79 cells and human colon carcinoma Caco-2 cells. Cytotoxicity was evaluated by the trypan blue exclusion technique in Caco-2 cells and by the plating efficiency assay in V79 cells. NMOR caused a dose-dependent decline of viable cells in both cell lines; MNNG induced a dose-dependent cytotoxic effect only in V79 cells. Pretreatment of cells with vitamin C and vitamin E significantly reduced the cytotoxicity of NMOR, however, both vitamins had not effect on cytotoxicity induced by MNNG. These results suggest that different
N-nitroso compounds react differently with cellular macromolecules. Measurement of the level of NMOR-induced DNA strand breaks and alkali-labile sites in both cell types using the alkaline comet assay also indicates a protective effect of both vitamins against the genotoxic effects of NMOR.</description><identifier>ISSN: 0304-3835</identifier><identifier>EISSN: 1872-7980</identifier><identifier>DOI: 10.1016/S0304-3835(02)00056-3</identifier><identifier>PMID: 12175518</identifier><identifier>CODEN: CALEDQ</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Apoptosis ; Ascorbic Acid - pharmacology ; Biological and medical sciences ; Caco-2 Cells ; Carcinogenesis, carcinogens and anticarcinogens ; Carcinogens - toxicity ; Cricetinae ; Cytotoxicity ; Deoxyribonucleic acid ; DNA ; DNA - biosynthesis ; DNA Damage ; DNA methylation ; Dose-Response Relationship, Drug ; Ethanol ; Foods and miscellaneous ; Genotoxicity ; Humans ; Medical research ; Medical sciences ; Membrane filters ; Methylnitronitrosoguanidine - toxicity ; Mitochondrial DNA ; N-methyl- N′-nitro- N-nitrosoguanidine ; N-nitrosomorpholine ; Nitrosamines - toxicity ; Tumors ; Vitamin C ; Vitamin E ; Vitamin E - pharmacology</subject><ispartof>Cancer letters, 2002-08, Vol.182 (1), p.11-18</ispartof><rights>2002 Elsevier Science Ireland Ltd</rights><rights>2002 INIST-CNRS</rights><rights>Copyright Elsevier Limited Aug 8, 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c485t-bc9e663c058387bb9d320be6215eeff4809099a6150bda782552c581058a30ef3</citedby><cites>FETCH-LOGICAL-c485t-bc9e663c058387bb9d320be6215eeff4809099a6150bda782552c581058a30ef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13767580$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12175518$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ROBICHOVA, Sona</creatorcontrib><creatorcontrib>SLAMENOVA, Darina</creatorcontrib><title>Effects of vitamins C and E on cytotoxicity induced by N-nitroso compounds, N-nitrosomorpholine and N-methyl- N′-nitro- N-nitrosoguanidine in Caco-2 and V79 cell lines</title><title>Cancer letters</title><addtitle>Cancer Lett</addtitle><description>Since
N-nitroso compounds as strong carcinogens are closely related to food and nutrition, the cytotoxic effects of
N-nitrosomorpholine (NMOR) and
N-methyl-
N′-nitro-
N-nitrosoguanidine (MNNG) and their reduction by vitamins C and E were investigated in hamster V79 cells and human colon carcinoma Caco-2 cells. Cytotoxicity was evaluated by the trypan blue exclusion technique in Caco-2 cells and by the plating efficiency assay in V79 cells. NMOR caused a dose-dependent decline of viable cells in both cell lines; MNNG induced a dose-dependent cytotoxic effect only in V79 cells. Pretreatment of cells with vitamin C and vitamin E significantly reduced the cytotoxicity of NMOR, however, both vitamins had not effect on cytotoxicity induced by MNNG. These results suggest that different
N-nitroso compounds react differently with cellular macromolecules. Measurement of the level of NMOR-induced DNA strand breaks and alkali-labile sites in both cell types using the alkaline comet assay also indicates a protective effect of both vitamins against the genotoxic effects of NMOR.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Ascorbic Acid - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Caco-2 Cells</subject><subject>Carcinogenesis, carcinogens and anticarcinogens</subject><subject>Carcinogens - toxicity</subject><subject>Cricetinae</subject><subject>Cytotoxicity</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - biosynthesis</subject><subject>DNA Damage</subject><subject>DNA methylation</subject><subject>Dose-Response Relationship, Drug</subject><subject>Ethanol</subject><subject>Foods and miscellaneous</subject><subject>Genotoxicity</subject><subject>Humans</subject><subject>Medical research</subject><subject>Medical sciences</subject><subject>Membrane filters</subject><subject>Methylnitronitrosoguanidine - toxicity</subject><subject>Mitochondrial DNA</subject><subject>N-methyl- N′-nitro- N-nitrosoguanidine</subject><subject>N-nitrosomorpholine</subject><subject>Nitrosamines - toxicity</subject><subject>Tumors</subject><subject>Vitamin C</subject><subject>Vitamin E</subject><subject>Vitamin E - pharmacology</subject><issn>0304-3835</issn><issn>1872-7980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqFkcuKFDEUhoMoTtv6CEpABAWjuVSqUiuRpr3A0C68bEMqSTkZqpKeJDVYO1_HrY_kk5jqbuylq0D4_j855wPgMcGvCCb168-Y4QoxwfhzTF9gjHmN2B2wIqKhqGkFvgtW_5AL8CCl6wWqGn4fXBBKGs6JWIFf2763OicYenjrshqdT3ADlTdwC4OHes4hhx9OuzxD582krYHdDHfIuxxDClCHcR8mb9LL8-UY4v4qDM7bQ9MOjTZfzQOCuz8_fx8hdKa_T8o7s8DOw43SAdFD7FvTQm2HAS5F6SG416sh2Uencw2-vtt-2XxAl5_ef9y8vUS6EjyjTre2rpnGXDDRdF1rGMWdrSnh1vZ9JXCL21bVhOPOqEZQzqnmghReMWx7tgZPj737GG4mm7K8DlP05UlZMpzVpCorXQN-pHSZIEXby310o4qzJFguguRBkFy2LzGVB0GSldyTU_vUjdacUycjBXh2AlTSauij8tqlM8eauuECF-7NkbNlF7fORpm0s77ocbEIlSa4_3zlL2_Xra0</recordid><startdate>20020808</startdate><enddate>20020808</enddate><creator>ROBICHOVA, Sona</creator><creator>SLAMENOVA, Darina</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope></search><sort><creationdate>20020808</creationdate><title>Effects of vitamins C and E on cytotoxicity induced by N-nitroso compounds, N-nitrosomorpholine and N-methyl- N′-nitro- N-nitrosoguanidine in Caco-2 and V79 cell lines</title><author>ROBICHOVA, Sona ; SLAMENOVA, Darina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c485t-bc9e663c058387bb9d320be6215eeff4809099a6150bda782552c581058a30ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Ascorbic Acid - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Caco-2 Cells</topic><topic>Carcinogenesis, carcinogens and anticarcinogens</topic><topic>Carcinogens - toxicity</topic><topic>Cricetinae</topic><topic>Cytotoxicity</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA - biosynthesis</topic><topic>DNA Damage</topic><topic>DNA methylation</topic><topic>Dose-Response Relationship, Drug</topic><topic>Ethanol</topic><topic>Foods and miscellaneous</topic><topic>Genotoxicity</topic><topic>Humans</topic><topic>Medical research</topic><topic>Medical sciences</topic><topic>Membrane filters</topic><topic>Methylnitronitrosoguanidine - toxicity</topic><topic>Mitochondrial DNA</topic><topic>N-methyl- N′-nitro- N-nitrosoguanidine</topic><topic>N-nitrosomorpholine</topic><topic>Nitrosamines - toxicity</topic><topic>Tumors</topic><topic>Vitamin C</topic><topic>Vitamin E</topic><topic>Vitamin E - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ROBICHOVA, Sona</creatorcontrib><creatorcontrib>SLAMENOVA, Darina</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><jtitle>Cancer letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ROBICHOVA, Sona</au><au>SLAMENOVA, Darina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of vitamins C and E on cytotoxicity induced by N-nitroso compounds, N-nitrosomorpholine and N-methyl- N′-nitro- N-nitrosoguanidine in Caco-2 and V79 cell lines</atitle><jtitle>Cancer letters</jtitle><addtitle>Cancer Lett</addtitle><date>2002-08-08</date><risdate>2002</risdate><volume>182</volume><issue>1</issue><spage>11</spage><epage>18</epage><pages>11-18</pages><issn>0304-3835</issn><eissn>1872-7980</eissn><coden>CALEDQ</coden><abstract>Since
N-nitroso compounds as strong carcinogens are closely related to food and nutrition, the cytotoxic effects of
N-nitrosomorpholine (NMOR) and
N-methyl-
N′-nitro-
N-nitrosoguanidine (MNNG) and their reduction by vitamins C and E were investigated in hamster V79 cells and human colon carcinoma Caco-2 cells. Cytotoxicity was evaluated by the trypan blue exclusion technique in Caco-2 cells and by the plating efficiency assay in V79 cells. NMOR caused a dose-dependent decline of viable cells in both cell lines; MNNG induced a dose-dependent cytotoxic effect only in V79 cells. Pretreatment of cells with vitamin C and vitamin E significantly reduced the cytotoxicity of NMOR, however, both vitamins had not effect on cytotoxicity induced by MNNG. These results suggest that different
N-nitroso compounds react differently with cellular macromolecules. Measurement of the level of NMOR-induced DNA strand breaks and alkali-labile sites in both cell types using the alkaline comet assay also indicates a protective effect of both vitamins against the genotoxic effects of NMOR.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>12175518</pmid><doi>10.1016/S0304-3835(02)00056-3</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-3835 |
| ispartof | Cancer letters, 2002-08, Vol.182 (1), p.11-18 |
| issn | 0304-3835 1872-7980 |
| language | eng |
| recordid | cdi_proquest_journals_1505361438 |
| source | Elsevier |
| subjects | Animals Apoptosis Ascorbic Acid - pharmacology Biological and medical sciences Caco-2 Cells Carcinogenesis, carcinogens and anticarcinogens Carcinogens - toxicity Cricetinae Cytotoxicity Deoxyribonucleic acid DNA DNA - biosynthesis DNA Damage DNA methylation Dose-Response Relationship, Drug Ethanol Foods and miscellaneous Genotoxicity Humans Medical research Medical sciences Membrane filters Methylnitronitrosoguanidine - toxicity Mitochondrial DNA N-methyl- N′-nitro- N-nitrosoguanidine N-nitrosomorpholine Nitrosamines - toxicity Tumors Vitamin C Vitamin E Vitamin E - pharmacology |
| title | Effects of vitamins C and E on cytotoxicity induced by N-nitroso compounds, N-nitrosomorpholine and N-methyl- N′-nitro- N-nitrosoguanidine in Caco-2 and V79 cell lines |
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