Enhancement of cytotoxic effect of bleomycin with transient permeabilization of plasma membrane by laser-induced multiple stress waves in vitro

We investigated the effect of multiple stress waves with peak stress of less than 3 MPa on chemosensitivity of HeLa cells adhered on plastic. HeLa cells exposed to stress waves retained more than 95% of the viability found in untreated cells. The scanning electron microscopy of cells exposed to stre...

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Bibliographic Details
Published in:Cancer letters 2003-09, Vol.199 (1), p.45-51
Main Authors: Miyamoto, Yuuichi, Umebayashi, Yukihiro, Koyano, Mikio, Wakita, Masayoshi, Nishisaka, Tsuyoshi
Format: Article
Language:English
Subjects:
Antimetabolites, Antineoplastic - toxicity
Antineoplastic agents
Biological and medical sciences
Bleomycin
Bleomycin - toxicity
Cancer
Cell Adhesion - drug effects
Cell Membrane Permeability - drug effects
Cell Membrane Permeability - physiology
Cell Membrane Permeability - radiation effects
Cell Survival - drug effects
Cytoplasm
Cytotoxicity
Drug delivery
HeLa Cells - drug effects
HeLa Cells - ultrastructure
Humans
Lasers
Medical sciences
Methods
Microscopy, Electron, Scanning
Microvilli - drug effects
Microvilli - ultrastructure
Pharmacology. Drug treatments
Plasma
Polyethylene terephthalate
Scanning electron microscopy
Shock waves
Stress waves
Stress, Mechanical
Studies
Transient permeabilization
Tumors
Ultrasonic imaging
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Summary:We investigated the effect of multiple stress waves with peak stress of less than 3 MPa on chemosensitivity of HeLa cells adhered on plastic. HeLa cells exposed to stress waves retained more than 95% of the viability found in untreated cells. The scanning electron microscopy of cells exposed to stress waves showed ruffling microvilli, indicating a change in the cell surface morphology. The cytotoxicity of bleomycin (BLM) on HeLa cells was enhanced by the stress waves exposure. Our findings demonstrated that the low-intensity stress wave would allow to deliver the BLM molecules into cytoplasm by repetition exposure.
ISSN:0304-3835
1872-7980
DOI:10.1016/S0304-3835(03)00332-X