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GST profiling may be useful in the screening for thyroid nodule malignancy
Screening tools are of utmost necessity in order to identify individuals at risk for thyroid nodule cancer. The polymorphic inheritance of human drug-metabolizing enzymes, such as those encoded by the Glutathione-S-Transferase (GST) system, plays an important role in the development of most human ca...
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| Published in: | Cancer letters 2004-06, Vol.209 (2), p.129-137 |
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| creator | Granja, Fabiana Morari, Joseane Morari, Elaine C Correa, Luiz A.C Assumpção, Lı́gia V.M Ward, Laura S |
| description | Screening tools are of utmost necessity in order to identify individuals at risk for thyroid nodule cancer. The polymorphic inheritance of human drug-metabolizing enzymes, such as those encoded by the Glutathione-S-Transferase (GST) system, plays an important role in the development of most human cancers. GSTP1 enzyme is the most important detoxification enzyme in human head and neck tissues. An aminoacid substitution (1105V) in the
GSTP1 gene result in two genotypes,
GSTP1AB and GSTP1BB. Those produce a variant enzyme with lower activity and less capability of effective detoxification of carcinogens than the wild type GSTP1AA. In order to look for the influence of GSTP1 enzymes inheritance pattern on thyroid cancer risk we used a PCR-SSCP-sequencing approach to compare the genotypes of 98 malignant nodules, including 77 papillary carcinomas (PC) and 21 follicular carcinomas (FC), to 44 benign nodules and to 157 healthy control individuals. Individuals with history of previous thyroid disease, exposure to radiation and antecedents of malignancy were excluded. Patients with PC and FC showed a significant over-representation of the variants of
GSTP1 allele compared to the control population (
p |
| doi_str_mv | 10.1016/j.canlet.2003.12.013 |
| format | article |
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GSTP1 gene result in two genotypes,
GSTP1AB and GSTP1BB. Those produce a variant enzyme with lower activity and less capability of effective detoxification of carcinogens than the wild type GSTP1AA. In order to look for the influence of GSTP1 enzymes inheritance pattern on thyroid cancer risk we used a PCR-SSCP-sequencing approach to compare the genotypes of 98 malignant nodules, including 77 papillary carcinomas (PC) and 21 follicular carcinomas (FC), to 44 benign nodules and to 157 healthy control individuals. Individuals with history of previous thyroid disease, exposure to radiation and antecedents of malignancy were excluded. Patients with PC and FC showed a significant over-representation of the variants of
GSTP1 allele compared to the control population (
p<0.0001). The risk for thyroid cancer in individuals with the variant GSTP1 enzymes, after adjusting for gender, age, tobacco and drugs use, increased 7,092 (CI: 2,307-21,802) and 9,625 (CI: 2.484-37.291) times for PC and FC, respectively. We suggest that
GST genotype may be associated with an increased susceptibility to thyroid cancer.
GSTP1 profiling from peripheral blood may be a simple and useful tool in the screening for thyroid nodule malignancy. Glutathione-S-Transferase system; GSTP; Thyroid cancer; Screening.</description><identifier>ISSN: 0304-3835</identifier><identifier>EISSN: 1872-7980</identifier><identifier>DOI: 10.1016/j.canlet.2003.12.013</identifier><identifier>PMID: 15159014</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Adenocarcinoma, Follicular - enzymology ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Biomarkers, Tumor - genetics ; Breast cancer ; Carcinoma, Papillary - enzymology ; Case-Control Studies ; CI, confidence interval ; Colleges & universities ; ECM, Elaine Cristina Morari ; F, Fisher test ; FC, follicular carcinomas ; Female ; Follow-Up Studies ; Gene Expression Profiling ; Genetic Predisposition to Disease - genetics ; Genotype ; Glutathione S-Transferase pi ; Glutathione Transferase - genetics ; GST, glutathione S-transferase ; GSTP1, glutathione S-transferase pi 1 ; GSTP1, glutathione S-transferase pi 1 locus ; Humans ; Isoenzymes - genetics ; Kruskal–Wallis, OR ; LSW, Laura Sterian Ward ; LVMA, Ligia Vera Montalli Assumpção ; M, mu ; Male ; Mass Screening ; Middle Aged ; Odds ratio ; PC, papillary carcinomas ; PCR, polymerase chain reaction ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Polymorphism, Single-Stranded Conformational ; Risk Factors ; SAS, statistical analysis system ; SSCP, single strand conformation polymorphism analysis ; T, theta ; Tg, thyroglobulin ; Thyroid cancer ; Thyroid Neoplasms - enzymology ; Thyroid Nodule - enzymology ; TSH, thyrotropin stimulating hormone ; Tumors ; χ2, Chi-square test</subject><ispartof>Cancer letters, 2004-06, Vol.209 (2), p.129-137</ispartof><rights>2004 Elsevier Ltd</rights><rights>Copyright 2004 Elsevier Ltd.</rights><rights>Copyright Elsevier Limited Jun 25, 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-e61346ba5e5333bc48c763dfb55b370c80786b76b55e27d3a9fb11638abadc903</citedby><cites>FETCH-LOGICAL-c386t-e61346ba5e5333bc48c763dfb55b370c80786b76b55e27d3a9fb11638abadc903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15159014$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Granja, Fabiana</creatorcontrib><creatorcontrib>Morari, Joseane</creatorcontrib><creatorcontrib>Morari, Elaine C</creatorcontrib><creatorcontrib>Correa, Luiz A.C</creatorcontrib><creatorcontrib>Assumpção, Lı́gia V.M</creatorcontrib><creatorcontrib>Ward, Laura S</creatorcontrib><title>GST profiling may be useful in the screening for thyroid nodule malignancy</title><title>Cancer letters</title><addtitle>Cancer Lett</addtitle><description>Screening tools are of utmost necessity in order to identify individuals at risk for thyroid nodule cancer. The polymorphic inheritance of human drug-metabolizing enzymes, such as those encoded by the Glutathione-S-Transferase (GST) system, plays an important role in the development of most human cancers. GSTP1 enzyme is the most important detoxification enzyme in human head and neck tissues. An aminoacid substitution (1105V) in the
GSTP1 gene result in two genotypes,
GSTP1AB and GSTP1BB. Those produce a variant enzyme with lower activity and less capability of effective detoxification of carcinogens than the wild type GSTP1AA. In order to look for the influence of GSTP1 enzymes inheritance pattern on thyroid cancer risk we used a PCR-SSCP-sequencing approach to compare the genotypes of 98 malignant nodules, including 77 papillary carcinomas (PC) and 21 follicular carcinomas (FC), to 44 benign nodules and to 157 healthy control individuals. Individuals with history of previous thyroid disease, exposure to radiation and antecedents of malignancy were excluded. Patients with PC and FC showed a significant over-representation of the variants of
GSTP1 allele compared to the control population (
p<0.0001). The risk for thyroid cancer in individuals with the variant GSTP1 enzymes, after adjusting for gender, age, tobacco and drugs use, increased 7,092 (CI: 2,307-21,802) and 9,625 (CI: 2.484-37.291) times for PC and FC, respectively. We suggest that
GST genotype may be associated with an increased susceptibility to thyroid cancer.
GSTP1 profiling from peripheral blood may be a simple and useful tool in the screening for thyroid nodule malignancy. Glutathione-S-Transferase system; GSTP; Thyroid cancer; Screening.</description><subject>Adenocarcinoma, Follicular - enzymology</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Breast cancer</subject><subject>Carcinoma, Papillary - enzymology</subject><subject>Case-Control Studies</subject><subject>CI, confidence interval</subject><subject>Colleges & universities</subject><subject>ECM, Elaine Cristina Morari</subject><subject>F, Fisher test</subject><subject>FC, follicular carcinomas</subject><subject>Female</subject><subject>Follow-Up Studies</subject><subject>Gene Expression Profiling</subject><subject>Genetic Predisposition to Disease - genetics</subject><subject>Genotype</subject><subject>Glutathione S-Transferase pi</subject><subject>Glutathione Transferase - genetics</subject><subject>GST, glutathione S-transferase</subject><subject>GSTP1, glutathione S-transferase pi 1</subject><subject>GSTP1, glutathione S-transferase pi 1 locus</subject><subject>Humans</subject><subject>Isoenzymes - genetics</subject><subject>Kruskal–Wallis, OR</subject><subject>LSW, Laura Sterian Ward</subject><subject>LVMA, Ligia Vera Montalli Assumpção</subject><subject>M, mu</subject><subject>Male</subject><subject>Mass Screening</subject><subject>Middle Aged</subject><subject>Odds ratio</subject><subject>PC, papillary carcinomas</subject><subject>PCR, polymerase chain reaction</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Genetic</subject><subject>Polymorphism, Single-Stranded Conformational</subject><subject>Risk Factors</subject><subject>SAS, statistical analysis system</subject><subject>SSCP, single strand conformation polymorphism analysis</subject><subject>T, theta</subject><subject>Tg, thyroglobulin</subject><subject>Thyroid cancer</subject><subject>Thyroid Neoplasms - enzymology</subject><subject>Thyroid Nodule - enzymology</subject><subject>TSH, thyrotropin stimulating hormone</subject><subject>Tumors</subject><subject>χ2, Chi-square test</subject><issn>0304-3835</issn><issn>1872-7980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNp9kMtKxDAUhoMozjj6BiIF160nTZN0NoKIVwQX6jok6alm6KRj0gp9ezPMgDtXITn_5eQj5JxCQYGKq1Vhte9wKEoAVtCyAMoOyJzWsszlsoZDMgcGVc5qxmfkJMYVAPBK8mMyo5zyJdBqTp4f3t6zTehb1zn_ma31lBnMxojt2GXOZ8MXZtEGRL8dt31IL1PoXZP5vhk7TI7OfXrt7XRKjlrdRTzbnwvycX_3fvuYv7w-PN3evOSW1WLIUVBWCaM5csaYsVVtpWBNazg3TIKtQdbCSJHuWMqG6WVrKBWs1kY3dglsQS53uWnt7xHjoFb9GHyqVJQDZ0LSFLgg1U5lQx9jwFZtglvrMCkKagtQrdQOoNoCVLRUCWCyXezDR7PG5s-0J5YE1zsBpi_-OAwqWofeYuMC2kE1vfu_4RcTLoJ2</recordid><startdate>20040625</startdate><enddate>20040625</enddate><creator>Granja, Fabiana</creator><creator>Morari, Joseane</creator><creator>Morari, Elaine C</creator><creator>Correa, Luiz A.C</creator><creator>Assumpção, Lı́gia V.M</creator><creator>Ward, Laura S</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope></search><sort><creationdate>20040625</creationdate><title>GST profiling may be useful in the screening for thyroid nodule malignancy</title><author>Granja, Fabiana ; Morari, Joseane ; Morari, Elaine C ; Correa, Luiz A.C ; Assumpção, Lı́gia V.M ; Ward, Laura S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-e61346ba5e5333bc48c763dfb55b370c80786b76b55e27d3a9fb11638abadc903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adenocarcinoma, Follicular - enzymology</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Breast cancer</topic><topic>Carcinoma, Papillary - enzymology</topic><topic>Case-Control Studies</topic><topic>CI, confidence interval</topic><topic>Colleges & universities</topic><topic>ECM, Elaine Cristina Morari</topic><topic>F, Fisher test</topic><topic>FC, follicular carcinomas</topic><topic>Female</topic><topic>Follow-Up Studies</topic><topic>Gene Expression Profiling</topic><topic>Genetic Predisposition to Disease - genetics</topic><topic>Genotype</topic><topic>Glutathione S-Transferase pi</topic><topic>Glutathione Transferase - genetics</topic><topic>GST, glutathione S-transferase</topic><topic>GSTP1, glutathione S-transferase pi 1</topic><topic>GSTP1, glutathione S-transferase pi 1 locus</topic><topic>Humans</topic><topic>Isoenzymes - genetics</topic><topic>Kruskal–Wallis, OR</topic><topic>LSW, Laura Sterian Ward</topic><topic>LVMA, Ligia Vera Montalli Assumpção</topic><topic>M, mu</topic><topic>Male</topic><topic>Mass Screening</topic><topic>Middle Aged</topic><topic>Odds ratio</topic><topic>PC, papillary carcinomas</topic><topic>PCR, polymerase chain reaction</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Genetic</topic><topic>Polymorphism, Single-Stranded Conformational</topic><topic>Risk Factors</topic><topic>SAS, statistical analysis system</topic><topic>SSCP, single strand conformation polymorphism analysis</topic><topic>T, theta</topic><topic>Tg, thyroglobulin</topic><topic>Thyroid cancer</topic><topic>Thyroid Neoplasms - enzymology</topic><topic>Thyroid Nodule - enzymology</topic><topic>TSH, thyrotropin stimulating hormone</topic><topic>Tumors</topic><topic>χ2, Chi-square test</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Granja, Fabiana</creatorcontrib><creatorcontrib>Morari, Joseane</creatorcontrib><creatorcontrib>Morari, Elaine C</creatorcontrib><creatorcontrib>Correa, Luiz A.C</creatorcontrib><creatorcontrib>Assumpção, Lı́gia V.M</creatorcontrib><creatorcontrib>Ward, Laura S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><jtitle>Cancer letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Granja, Fabiana</au><au>Morari, Joseane</au><au>Morari, Elaine C</au><au>Correa, Luiz A.C</au><au>Assumpção, Lı́gia V.M</au><au>Ward, Laura S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GST profiling may be useful in the screening for thyroid nodule malignancy</atitle><jtitle>Cancer letters</jtitle><addtitle>Cancer Lett</addtitle><date>2004-06-25</date><risdate>2004</risdate><volume>209</volume><issue>2</issue><spage>129</spage><epage>137</epage><pages>129-137</pages><issn>0304-3835</issn><eissn>1872-7980</eissn><abstract>Screening tools are of utmost necessity in order to identify individuals at risk for thyroid nodule cancer. The polymorphic inheritance of human drug-metabolizing enzymes, such as those encoded by the Glutathione-S-Transferase (GST) system, plays an important role in the development of most human cancers. GSTP1 enzyme is the most important detoxification enzyme in human head and neck tissues. An aminoacid substitution (1105V) in the
GSTP1 gene result in two genotypes,
GSTP1AB and GSTP1BB. Those produce a variant enzyme with lower activity and less capability of effective detoxification of carcinogens than the wild type GSTP1AA. In order to look for the influence of GSTP1 enzymes inheritance pattern on thyroid cancer risk we used a PCR-SSCP-sequencing approach to compare the genotypes of 98 malignant nodules, including 77 papillary carcinomas (PC) and 21 follicular carcinomas (FC), to 44 benign nodules and to 157 healthy control individuals. Individuals with history of previous thyroid disease, exposure to radiation and antecedents of malignancy were excluded. Patients with PC and FC showed a significant over-representation of the variants of
GSTP1 allele compared to the control population (
p<0.0001). The risk for thyroid cancer in individuals with the variant GSTP1 enzymes, after adjusting for gender, age, tobacco and drugs use, increased 7,092 (CI: 2,307-21,802) and 9,625 (CI: 2.484-37.291) times for PC and FC, respectively. We suggest that
GST genotype may be associated with an increased susceptibility to thyroid cancer.
GSTP1 profiling from peripheral blood may be a simple and useful tool in the screening for thyroid nodule malignancy. Glutathione-S-Transferase system; GSTP; Thyroid cancer; Screening.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>15159014</pmid><doi>10.1016/j.canlet.2003.12.013</doi><tpages>9</tpages></addata></record> |
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| ispartof | Cancer letters, 2004-06, Vol.209 (2), p.129-137 |
| issn | 0304-3835 1872-7980 |
| language | eng |
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| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Adenocarcinoma, Follicular - enzymology Adolescent Adult Aged Aged, 80 and over Biomarkers, Tumor - genetics Breast cancer Carcinoma, Papillary - enzymology Case-Control Studies CI, confidence interval Colleges & universities ECM, Elaine Cristina Morari F, Fisher test FC, follicular carcinomas Female Follow-Up Studies Gene Expression Profiling Genetic Predisposition to Disease - genetics Genotype Glutathione S-Transferase pi Glutathione Transferase - genetics GST, glutathione S-transferase GSTP1, glutathione S-transferase pi 1 GSTP1, glutathione S-transferase pi 1 locus Humans Isoenzymes - genetics Kruskal–Wallis, OR LSW, Laura Sterian Ward LVMA, Ligia Vera Montalli Assumpção M, mu Male Mass Screening Middle Aged Odds ratio PC, papillary carcinomas PCR, polymerase chain reaction Polymerase Chain Reaction Polymorphism, Genetic Polymorphism, Single-Stranded Conformational Risk Factors SAS, statistical analysis system SSCP, single strand conformation polymorphism analysis T, theta Tg, thyroglobulin Thyroid cancer Thyroid Neoplasms - enzymology Thyroid Nodule - enzymology TSH, thyrotropin stimulating hormone Tumors χ2, Chi-square test |
| title | GST profiling may be useful in the screening for thyroid nodule malignancy |
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