A novel amplification target, ARHGAP5 , promotes cell spreading and migration by negatively regulating RhoA in Huh-7 hepatocellular carcinoma cells

Abstract RhoA, a member of the Rho family of small GTPases, directs the organization of the actin cytoskeleton and is involved in regulating cell shape and movement. Its activity is negatively regulated by p190-B RhoGAP (GTPase-activating protein). We investigated DNA copy number aberrations in huma...

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Bibliographic Details
Published in:Cancer letters 2009-03, Vol.275 (1), p.27-34
Main Authors: Gen, Yasuyuki, Yasui, Kohichiroh, Zen, Keika, Nakajima, Tomoaki, Tsuji, Kazuhiro, Endo, Mio, Mitsuyoshi, Hironori, Minami, Masahito, Itoh, Yoshito, Tanaka, Shinji, Taniwaki, Masafumi, Arii, Shigeki, Okanoue, Takeshi, Yoshikawa, Toshikazu
Format: Article
Language:English
Subjects:
Amplification
ARHGAP5
Artificial chromosomes
Carcinoma, Hepatocellular - metabolism
Cell Line, Tumor
Cell Movement
Chromosomes, Human, Pair 14 - ultrastructure
Cytoskeleton
Cytoskeleton - metabolism
Deoxyribonucleic acid
Disease Progression
DNA
DNA - metabolism
Enzymes
Gene expression
Gene Expression Regulation, Neoplastic
Genomes
GTPase-Activating Proteins - metabolism
Hematology, Oncology and Palliative Medicine
Hepatocellular carcinoma
Humans
In Situ Hybridization, Fluorescence
Liver cancer
Liver Neoplasms - metabolism
Migration
p190-B RhoGAP
Proteins
Reverse Transcriptase Polymerase Chain Reaction
RhoA
rhoA GTP-Binding Protein - metabolism
RNA Interference
Studies
Tumors
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Summary:Abstract RhoA, a member of the Rho family of small GTPases, directs the organization of the actin cytoskeleton and is involved in regulating cell shape and movement. Its activity is negatively regulated by p190-B RhoGAP (GTPase-activating protein). We investigated DNA copy number aberrations in human hepatocellular carcinoma and esophageal squamous cell carcinoma cell lines using a high-density oligonucleotide microarray and found a novel amplification at chromosomal region 14q12. We identified ARHGAP5 (the gene encoding p190-B RhoGAP) as a probable target for the amplification at 14q12, and our results showed that p190-B RhoGAP promotes cells spreading and migration by negatively regulating RhoA activity in Huh-7 hepatocellular carcinoma cells.
ISSN:0304-3835
1872-7980
DOI:10.1016/j.canlet.2008.09.036