Comparison of human plasma arterial input function for [11C]MePPEP determined with LC-MS/MS and radiometric methods

The purpose of this work was to compare LC-MS/MS and conventional radiometric methods for determining the arterial input function of [11C]MePPEP, a CB1 receptor radioligand, in human plasma. Methods:In vitro experiment: [11C]MePPEP (5µCi; 7pmol) was added to human blood (10mL) and six plasma samples...

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Bibliographic Details
Published in:NeuroImage (Orlando, Fla.) Fla.), 2010-08, Vol.52 (S1), p.S162-S162
Main Authors: Pike, Victor W., Zoghbi, Sami S., Shetty, H. Umesha, Hirvonen, Jussi, Anderson, Kacey B., Jenko, Kimberly J., Morse, Cheryl, Innis, Robert B.
Format: Article
Language:English
Subjects:
Methods
Plasma
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Summary:The purpose of this work was to compare LC-MS/MS and conventional radiometric methods for determining the arterial input function of [11C]MePPEP, a CB1 receptor radioligand, in human plasma. Methods:In vitro experiment: [11C]MePPEP (5µCi; 7pmol) was added to human blood (10mL) and six plasma samples (200µL) were isolated for both radiometric and LC-MS/MS analyses.
ISSN:1053-8119
1095-9572
DOI:10.1016/j.neuroimage.2010.04.132