DEVELOPMENT AND VALIDATION OF A STABILITY-INDICATING RP-LC METHOD FOR THE DETERMINATION OF ANTICANCER DRUG EPIRUBICIN IN PHARMACEUTICALS

In the present paper, sensitive, rapid, and different analytical methodology was developed for the determination of anticancer drug epirubicin (EPR). The mixture of epirubicin and moxifloxacin as internal standard was separated on a reversed phase Waters Spherisorb ODS1 column (250mm × 4.6mm × 5mm)...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2014-07, Vol.37 (11), p.1583-1596
Main Authors: Kurbanoglu, Sevinc, Palabiyik, Burcin Bozal, Gumustas, Mehmet, Şanlı, Senem, Uslu, Bengi, Ozkan, Sibel A.
Format: Article
Language:English
Subjects:
determination
epirubicin
liquid chromatography
stability-indicating high-performance
validation
Online Access:Get full text
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Summary:In the present paper, sensitive, rapid, and different analytical methodology was developed for the determination of anticancer drug epirubicin (EPR). The mixture of epirubicin and moxifloxacin as internal standard was separated on a reversed phase Waters Spherisorb ODS1 column (250mm × 4.6mm × 5mm) using acetonitrile/water (30:70 v/v) mixture containing 15 mM phosphoric acid as mobile phase at 0.6 mL min −1 flow rate and 30 C. Also degradation studies were conducted as stress conditions of UV light, acidic hydrolysis, alkaline hydrolysis, oxidation, and heat in oven (100°C), to evaluate the ability of the proposed method for the separation of EPR from its degradation products. The validated method suggests routine analysis of EPR in differently equipped laboratories.
ISSN:1082-6076
1520-572X
DOI:10.1080/10826076.2013.803202