Lactobacillus gasseriK7 modulates the blood cell transcriptome of conventional mice infected with Escherichia coliO157:H7

• Published in: Journal of applied microbiology 2014-05, Vol.116 (5), p.1282
• Main Authors: Sagaya, FM, Hacin, B, Tompa, G, Ihan, A, Spela, S, Cerne, M, Hurrell, RF, Matijasic, BB, Rogelj, I, Vergeres, G
• Format: Article
• Language: English
• Subjects: Biomarkers; E coli; Microbiology; Probiotics; Rodents
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.12440

Aims As the immune cells underlying the intestinal barrier sense luminal microbial signals, blood cell transcriptomics may identify subclinical changes triggered by gut bacteria that may otherwise not be detected. We have therefore investigated how Lactobacillus gasseriK7 and enterohemorrhagic Escherichia coliO157:H7 modulate the blood cell transcriptome of mice possessing an intact microbiota. Methods and Results We have analysed the transcriptome of five groups of C57BL/6J mice: (i) control, (ii) inoculated with a single dose of E. coli, (iii) inoculated during 2 weeks with Lact. gasseri, (iv) co-inoculated with E. coli and Lact. gasseri, (v) inoculated with Lact. gasseri prior to E. coli infection. The transcriptome could distinguish between the five treatment groups. Gene characteristics of bacterial infection, in particular inflammation, were upregulated in the mice inoculated with E. coli. Lact. gasseri had only mild effects on the transcriptome but modified the gene expression induced by E. coli. Conclusions The transcriptome differentiates mice inoculated orally with E. coli, Lact. gasseri and combinations of these two strains. Significance and Impact of the Study These results suggest that the blood cell transcriptome can be used as a source of biomarkers to monitor the impact of probiotics in subclinical models of infectious disease. [PUBLICATION ABSTRACT]