GRP78 expression and regulation in the mouse uterus during embryo implantation

The aim of this study was to investigate the spatiotemporal expression and regulation of GRP78 in the mouse uterus during the peri-implantation period. The GRP78 protein was mainly detected in the luminal and glandular epithelia on days 1–4 of pregnancy. On day 5 of pregnancy, the GRP78 protein was...

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Bibliographic Details
Published in:Journal of molecular histology 2014-06, Vol.45 (3), p.259-268
Main Authors: Lin, PengFei, Jin, YaPing, Lan, XiangLi, Yang, YanZhou, Chen, Fenglei, Wang, Nan, Li, Xiao, Sun, YuJie, Wang, AiHua
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Cell Biology
Developmental Biology
Embryo Implantation - genetics
Female
Gene Expression Regulation, Developmental - drug effects
Gonadal Steroid Hormones - metabolism
Gonadal Steroid Hormones - pharmacology
Heat-Shock Proteins - genetics
Heat-Shock Proteins - metabolism
Immunohistochemistry
In Situ Hybridization
Life Sciences
Mice
Original Paper
Ovary - metabolism
Pregnancy
Pseudopregnancy - genetics
Pseudopregnancy - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
Uterus - metabolism
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Summary:The aim of this study was to investigate the spatiotemporal expression and regulation of GRP78 in the mouse uterus during the peri-implantation period. The GRP78 protein was mainly detected in the luminal and glandular epithelia on days 1–4 of pregnancy. On day 5 of pregnancy, the GRP78 protein was more highly observed around the implanted embryo at the implantation site. There was no detectable GRP78 protein signal on day 5 of pseudopregnancy. GRP78 mRNA and protein levels gradually increased on days 6–8 of pregnancy, and the expression pattern was also expanded, coinciding with the development of decidua. Similarly, GRP78 expression was also strongly expressed in decidualised cells following artificial decidualisation. Compared with the results obtained with the delayed uterus, a high level of GRP78 expression was detected in the implantation-activated uterus. In the uteri of ovariectomised mice, GRP78 expression increased and reached its highest level after injection of oestrogen, and progesterone seemed to have an antagonistic effect on oestrogen up-regulation of GRP78 expression. Our data indicate that GRP78 might play an important role during the process of mouse embryo implantation, and GRP78 expression was mainly regulated by active blastocysts and maternal oestrogen.
ISSN:1567-2379
1567-2387
DOI:10.1007/s10735-013-9552-1