Evaluation of the methylation profile of exfoliated cell samples from patients with head and neck squamous cell carcinoma

ABSTRACT Background Silencing of tumor suppressor genes plays a vital role in head and neck carcinogenesis. The purposes of this study were to determine the methylation profile of exfoliated tumors cells collected from patients with head and neck squamous cell carcinoma (HNSCC) and to evaluate its p...

Full description

Saved in:
Bibliographic Details
Published in:Head & neck 2014-05, Vol.36 (5), p.631-637
Main Authors: Longo, Ana Luiza Bomfim, Rettori, Marianna M., de Carvalho, Ana Carolina, Kowalski, Luiz Paulo, Carvalho, Andre Lopes, Vettore, Andre Luiz
Format: Article
Language:English
Subjects:
Aged
Carcinoma, Squamous Cell - genetics
Carcinoma, Squamous Cell - mortality
Carcinoma, Squamous Cell - pathology
Cohort Studies
Cyclin A1 - genetics
Disease-Free Survival
DNA Methylation
exfoliated cell
Female
Follow-Up Studies
Genes, DCC - genetics
Genes, Tumor Suppressor
Head and Neck Neoplasms - genetics
Head and Neck Neoplasms - mortality
Head and Neck Neoplasms - pathology
head and neck squamous cell carcinoma (HNSCC)
Humans
Kaplan-Meier Estimate
Male
methylation
Middle Aged
Polymerase Chain Reaction
Prospective Studies
qMSP
Risk Assessment
Squamous Cell Carcinoma of Head and Neck
Statistics, Nonparametric
Survival Rate
Tissue Inhibitor of Metalloproteinase-3 - genetics
Tumor Cells, Cultured
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:ABSTRACT Background Silencing of tumor suppressor genes plays a vital role in head and neck carcinogenesis. The purposes of this study were to determine the methylation profile of exfoliated tumors cells collected from patients with head and neck squamous cell carcinoma (HNSCC) and to evaluate its prognostic significance. Methods The methylation profile and level of a 20‐gene panel were evaluated by quantitative methylation‐specific polymerase chain reaction (qMSP) in exfoliated tumor cell samples from 96 patients with HNSCC. Results CCNA1 (60.4%), DCC (54.2%), and TIMP3 (35.4%) were frequently methylated in these samples. Patients with exfoliated tumors cells positive for DCC methylation showed a trend toward a lower local recurrence‐free survival. Conclusion These findings indicate that a low invasive method could be used to access the methylation profile of exfoliated cells from patients with HNSCC. Moreover, our data provide evidence that hypermethylation of DCC could be useful as prognostic indicator for this malignancy. © 2013 Wiley Periodicals, Inc. Head Neck 36: 631–637, 2014
ISSN:1043-3074
1097-0347
DOI:10.1002/hed.23345