Algae Biomass Based Media for Poly(3-hydroxybutyrate) (PHB) Production by Escherichia coli

In addition to biodiesel production from algae, the production of other valuable bioproducts facilitates the development of an algae-based biorefinery platform. The goal of this study was to utilize the aqueous fraction from a novel algal wet lipid extraction procedure as the medium for the producti...

Full description

Saved in:
Bibliographic Details
Published in:Journal of polymers and the environment 2014-06, Vol.22 (2), p.272-277
Main Authors: Sathish, Ashik, Glaittli, Katherine, Sims, Ronald C., Miller, Charles D.
Format: Article
Language:English
Subjects:
Algae
Applied sciences
Bioconversions. Hemisynthesis
Biological and medical sciences
Biomass
Bioplastics
Biorefineries
Biotechnology
Brief Communication
Chemistry
Chemistry and Materials Science
E coli
Environmental Chemistry
Environmental Engineering/Biotechnology
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Industrial Chemistry/Chemical Engineering
Materials Science
Methods. Procedures. Technologies
Organic polymers
Physicochemistry of polymers
Poly-3-hydroxybutyrate
Polycondensation
Polymer Sciences
Preparation, kinetics, thermodynamics, mechanism and catalysts
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In addition to biodiesel production from algae, the production of other valuable bioproducts facilitates the development of an algae-based biorefinery platform. The goal of this study was to utilize the aqueous fraction from a novel algal wet lipid extraction procedure as the medium for the production of a bio product, poly(3-hydroxybutyrate) (PHB), via the growth of recombinant Escherichia coli. PHB yield was measured at 34 % of the E. coli dry cell mass, and was increased to 51 % when the algae aqueous medium was supplemented with glucose. While the addition of inorganic nutrients to the aqueous phase did not increase PHB production or growth of E. coli , growth of E. coli was observed to increase with the supplementation of carbon substrate (glucose). The addition of carbon rich waste to the aqueous fraction of wastewater-derived algae may in the future provide a sustainable alternative. Future research will be directed at evaluating this concept to develop a sustainable process for the production of bioplastics through an algae-based biorefinery platform.
ISSN:1566-2543
1572-8919
1572-8900
DOI:10.1007/s10924-014-0647-x