Occurrence of Groundnut ringspot virus on Brazilian peanut crops

A high incidence of plants with mosaic, chlorotic spots, ringspots, necrosis, smaller leaves, and stunting was observed on peanut crops (Arachis hypogaea L.) in Itápolis, São Paulo State, Brazil. Transmission electron microscope examination of thin sections of infected leaves revealed the presence o...

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Bibliographic Details
Published in:Journal of general plant pathology : JGPP 2014-05, Vol.80 (3), p.282-286
Main Authors: Camelo-García, Viviana Marcela, Lima, Élison Fabricio B, Mansilla-Córdova, Pedro Javier, Rezende, Jorge Alberto Marques, Kitajima, Elliot Watanabe, Barreto, Modesto
Format: Article
Language:English
Subjects:
Agriculture
amino acid sequences
Amino acids
Arachis hypogaea
Biomedical and Life Sciences
crops
Disease Note
DNA
genes
Groundnut ringspot virus
growth retardation
Leaves
Life Sciences
Microbiology
necrosis
nucleocapsid
Peanuts
Plant diseases
Plant Pathology
reverse transcriptase polymerase chain reaction
RNA
Transmission electron microscopy
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Summary:A high incidence of plants with mosaic, chlorotic spots, ringspots, necrosis, smaller leaves, and stunting was observed on peanut crops (Arachis hypogaea L.) in Itápolis, São Paulo State, Brazil. Transmission electron microscope examination of thin sections of infected leaves revealed the presence of spheroidal particles, ca. 80 nm in diameter, suggestive of Tospovirus. A DNA fragment of ~600 bp was amplified by RT-PCR from total RNA extracted from infected tissues using primers specific for the nucleocapsid gene of Groundnut ringspot virus (GRSV). Nucleotide and deduced amino acid sequences of the fragments showed high identities with known GRSV isolates.
ISSN:1345-2630
1610-739X
DOI:10.1007/s10327-014-0518-2