EFFECT OF ENZYMES ON GLUCOSE ISOMERIZATION NONLINEAR REACTION CHROMATOGRAPHY

Adsorption isotherms play an important role in preparation chromatography, which is the basis of procedure design and optimization. For a binary competitive system, the adsorption isotherm constant G is generally derived from linear chromatography. However, the adsorption isotherm coefficient b is d...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2014-10, Vol.37 (17), p.2389-2402
Main Authors: Wu, Xiuhong, Lin, Bingchang, Quan, Yanling, Zhu, PeiXun, Gao, Hong, Feng, Wen Qiang
Format: Article
Language:English
Subjects:
Adsorption
batch reactors
catalysis
chromatographic separations
Chromatography
Enzymes
thermodynamics
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Summary:Adsorption isotherms play an important role in preparation chromatography, which is the basis of procedure design and optimization. For a binary competitive system, the adsorption isotherm constant G is generally derived from linear chromatography. However, the adsorption isotherm coefficient b is difficult to measure, especially for separations accompanied by a reaction. In previous studies, the effect of enzymes has often been simplified or ignored due to its complexity. In the present paper, b was calculated using nonlinear reaction chromatography theory and the reaction rate constant k r . The obtained results were compared using several experimental methods, such as the elution method and the dynamic Michaelis-Menten method, the results of which were generally in agreement. The feasibility of the method was discussed. The method presented herein might provide a simple way to determine the adsorption isotherm of reaction chromatography when enzymes are involved.
ISSN:1082-6076
1520-572X
DOI:10.1080/10826076.2013.836711