Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the bovine CD8[alpha][alpha]-BoLA-202201 complex

• Published in: Acta crystallographica. Section F, Structural biology communications Structural biology communications, 2014-06, Vol.70 (6), p.742
• Main Authors: Wang, Zhenbao, Chen, Rong, Tariq, Mansoor, Jiang, Bo, Chen, Zhaosan, Xia, Chun
• Format: Article
• Language: English
• ISSN: 2053-230X
• DOI: 10.1107/S2053230X14008838

In order to clarify the structural characteristics of the bovine MHC class I molecule (BoLA-I) complexed with CD8[alpha][alpha] (CD8[alpha][alpha]-BoLA-I), bovine CD8[alpha][alpha], BoLA-I (BoLA-2*02201) and [beta]2m were expressed and purified, and were then assembled with a peptide derived from Foot-and-mouth disease virus (FMDV-VP1YY9) and crystallized. The crystal diffracted to 1.7Å resolution and belonged to space group P21, with unit-cell parameters a = 53.9, b = 103.8, c = 61.8Å, [alpha] = [gamma] = 90, [beta] = 96°. The asymmetric unit contained one complex, with a Matthews coefficient of 2.41Å3Da-1 and a solvent content of 48.9%. The rotation-function Z-score and translation-function Z-score for molecular replacement were 3.4 and 8.9, respectively. In addition, SDS-PAGE analysis of CD8[alpha][alpha]-BoLA-I crystals showed three bands corresponding to the molecular weights of BoLA-I heavy chain, [beta]2m and CD8[alpha]. The structure of the CD8[alpha][alpha]-BoLA-I complex should be helpful in obtaining insight into the interaction between bovine CD8[alpha][alpha] and MHC class I molecules. Structure determination of BoLA-2*02201-FMDV-VP1YY9 will be useful in the design of vaccines for foot-and-mouth disease. [PUBLICATION ABSTRACT]