Structure of d(CCCCGGTACCGGGG)2 at 1.65Å resolution

The crystal structure of the tetradecanucleotide d(CCCCGGTACCGGGG)2 has previously been reported as an A-type double helix at a resolution of 2.5Å in space group P41. Here, the structure of this sequence was determined at a significantly higher resolution of 1.65Å in space group P41212. The differen...

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Bibliographic Details
Published in:Acta crystallographica. Section F, Structural biology communications Structural biology communications, 2014-07, Vol.70 (7), p.860
Main Authors: Purushothaman, Monica, Varghese, Anna, Mandal, Pradeep Kumar, Gautham, Namasivayam
Format: Article
Language:English
Online Access:Get full text
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Summary:The crystal structure of the tetradecanucleotide d(CCCCGGTACCGGGG)2 has previously been reported as an A-type double helix at a resolution of 2.5Å in space group P41. Here, the structure of this sequence was determined at a significantly higher resolution of 1.65Å in space group P41212. The differences in crystal packing between the former and latter are described. The crystallographic asymmetric unit consists of one tetradecanucleotide duplex that spans more than one full turn of the A-helix. This structure allowed the unambiguous identification of solvent interactions. [PUBLICATION ABSTRACT]
ISSN:2053-230X
DOI:10.1107/S2053230X1401084X