Characterization of a peptide vaccine candidate mimicking an oligosaccharide epitope ofNeisseria gonorrhoeaeand resultant immune responses and function

The 2C7 epitope is a conserved oligosaccharide structure, a part of lipooligosaccharide (LOS) onNeisseria gonorrhoeae, present in 95% of clinical gonococcal isolates. 2C7 may represent a potential candidate for an anti-gonococcal vaccine. To circumvent the limitations of saccharide immunogens in pro...

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Bibliographic Details
Published in:Vaccine 2006-01, Vol.24 (2), p.157
Main Authors: Ngampasutadol, Jutamas, Rice, Peter A, Walsh, Mary T, Gulati, Sunita
Format: Article
Language:English
Subjects:
Bacteriology
Cloning
E coli
Gonorrhea
Immunization
Infections
Lactose
Peptides
Sexually transmitted diseases
STD
Vaccines
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Summary:The 2C7 epitope is a conserved oligosaccharide structure, a part of lipooligosaccharide (LOS) onNeisseria gonorrhoeae, present in 95% of clinical gonococcal isolates. 2C7 may represent a potential candidate for an anti-gonococcal vaccine. To circumvent the limitations of saccharide immunogens in producing long lived immune responses, we identified a peptide that mimics the 2C7 epitope using a random peptide library, characterizing linear and cyclic forms and formulating a multiple antigenic peptide. The multiple antigenic peptide Octa-MAP1 was used for immunization, and elicited >=4-fold increase in cross-reactive anti-LOS antibodies in 26 of 30 mice (87%). IgG anti-LOS antibody elicited by Octa-MAP1 immunization possessed dose-responsive direct complement (C)-dependent bactericidal activity against gonococcal strains that expressed the 2C7 epitope. These data indicate that a peptide can mimic an oligosaccharide epitope and may form the basis for the development of a vaccine candidate for human immunization againstN. gonorrhoeae.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2005.07.065