Induction of Th1-type cellular responses in cured/exposedLeishmania-infected patients and hamsters against polyproteins of solubleLeishmania donovanipromastigotes ranging from 89.9 to 97.1kDa

• Published in: Vaccine 2008-09, Vol.26 (37), p.4813
• Main Authors: Kumari, Shraddha, Samant, Mukesh, Khare, Prashant, Sundar, Shyam, Sinha, Sudhir, Dube, Anuradha
• Format: Article
• Language: English
• Subjects: Immune system; Lymphocytes; Parasites; Parasitic diseases; Vaccines; Vector-borne diseases
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2008.06.102

Earlier, we have identified soluble antigenic fraction ranging from 68 to 97.4kDa (F2-fraction) ofLeishmania donovanipromastigote, which induced Th1-immunostimulatory cellular responses in both curedLeishmaniapatients/hamsters and exhibited significant prophylactic potential against experimental visceral leishmaniasis (VL). In the present study, we have further fractionated F2-fraction by continuous elution SDS-PAGE and subjected them to re-evaluation for their ability to induce cellular responses. Out of seven sub-fractions: F2.1-F2.7, only four F2.4-F2.7 (89.9-97.1kDa), individually or in pooled sub-fractions (P4-7), stimulated Th1-type remarkable lymphoproliferative, NO, IFN-γ and IL-12 responses inLeishmania-infected cured/exposed patients and hamsters. Interestingly, optimum Th1-responses were obtained with P4-7 suggesting the presence of immunostimulatory molecules in it and may be exploited for developing a successful subunit vaccine against VL.