Cytotoxicity and Antioxidative Effects of Herbal and Fruit Extracts In Vitro

Many studies have been carried out on bioactivities of individual herbs/fruits using in cosmetics or as a diet products, however, no collective study on their comparative antioxidant activities against oxidative damage or on cytotoxicity effect has been reported. The aim of this work was study the c...

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Bibliographic Details
Published in:Food biophysics 2014-09, Vol.9 (3), p.267-276
Main Authors: Tomankova, Katerina, Kolarova, Hana, Pizova, Klara, Binder, Svatopluk, Konecny, Petr, Kriegova, Eva, Malina, Lukas, Horakova, Jana, Malohlava, Jakub, Kejlova, Kristina, Jirova, Dagmar
Format: Article
Language:English
Subjects:
Analytical Chemistry
antioxidant activity
Antioxidants
Bioassays
Biological and Medical Physics
Biophysics
cell cycle
cell death
cell viability
Chemistry
Chemistry and Materials Science
Cosmetics
Cytotoxicity
Deoxyribonucleic acid
DNA
DNA damage
fluorescence
Food Science
fruit extracts
Fruits
gene expression
herbs
membrane potential
mice
Original Article
Oxidative stress
Prunella vulgaris
reactive oxygen species
Ultraviolet radiation
wheat germ oil
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Summary:Many studies have been carried out on bioactivities of individual herbs/fruits using in cosmetics or as a diet products, however, no collective study on their comparative antioxidant activities against oxidative damage or on cytotoxicity effect has been reported. The aim of this work was study the cytotoxicity and antioxidative activity of eight extracts with hypothetical antioxidative influence in vitro. To further elucidate of a possible role of herbals/fruits extracts on cell protection was used on the healthy and UV-A damaged mouse fibroblast cells. The cell viability was detect using MTT assay. Kinetic production of reactive oxygen species, identification of cell death, cell cycle and gene expression C-FOS were measured. Intracellular and mitochondrial transmembrane potential was evaluate with JC-1 fluorescence probe. Comet assay was employed to detect the UV-A induced DNA damage. The results indicated that using the extracts decreased ROS production. It can lead to greatly enhance and promote the viability of cells. As the most effective antioxidant in quenching of the ROS, in cell viability and DNA presentation was determined Prunella Vulgaris. However, one of them (Wheat Germ Oil) caused increased production of ROS and low cell viability.
ISSN:1557-1858
1557-1866
DOI:10.1007/s11483-014-9349-0