Mycobacterium tuberculosisarabinomannan-protein conjugates protect against tuberculosis

Lipoarabinomannan (LAM) is a major structural surface component of mycobacteria. Arabinomannan (AM) oligosaccharides derived from LAM ofMycobacterium tuberculosisH37Rv were isolated and covalently conjugated to tetanus toxoid (TT) or to short-term culture filtrate proteins (antigen 85B (Ag85B) or a...

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Bibliographic Details
Published in:Vaccine 2003-09, Vol.21 (25-26), p.4081
Main Authors: Hamasur, Beston, Haile, Melles, Pawlowski, Andrzej, Schröder, Ulf, Williams, Ann, Hatch, Graham, Hall, Graham, Marsh, Philip, Källenius, Gunilla, Svenson, Stefan B
Format: Article
Language:English
Subjects:
Bacteria
Candidates
Carbohydrates
Cell growth
Chromatography
Filtrate
Immunization
Lipids
Molecular weight
Proteins
Survival
Tuberculosis
Vaccines
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Summary:Lipoarabinomannan (LAM) is a major structural surface component of mycobacteria. Arabinomannan (AM) oligosaccharides derived from LAM ofMycobacterium tuberculosisH37Rv were isolated and covalently conjugated to tetanus toxoid (TT) or to short-term culture filtrate proteins (antigen 85B (Ag85B) or a 75kDa protein) fromM. tuberculosisstrain Harlingen. The different AM oligosaccharide (AMOs)-protein conjugate vaccine candidates proved to be highly immunogenic, inducing boosterable IgG responses against the AMOs portion of the conjugates in rabbits and guinea-pigs. Proliferation of T-cells from C57BL/6 mice immunized with the conjugates was seen upon in vitro stimulation with PPD. In C57BL/6 mice subcutaneous immunization with the AMOs-antigen 85B conjugate in alum provided significant protection compared to sham (alum only) immunized mice (P
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(03)00274-3