Antimicrobial effect of the triterpene 3[beta],6[beta],16[beta]-trihydroxylup-20-ene on planktonic cells and biofilms from gram positive and gram negative bacteria

This study evaluated the antimicrobial effect of 3β,6β,16β-trihydroxylup-20(29)-ene (CLF1), a triterpene isolated from Combretum leprosum Mart., in inhibiting the planktonic growth and biofilms of Gram positive bacteria Streptococcus mutans and S. mitis. The antimicrobial activity was assessed by de...

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Bibliographic Details
Published in:BioMed research international 2014-01, Vol.2014
Main Authors: Evaristo, Francisco Flavio Vasconcelos, Albuquerque, Maria Rose Jane R, Santos, Helcio Silva dos, Bandeira, Paulo Nogueira, Avila, Fabio do Nascimento, da Silva, Bruno Rocha, Vasconcelos, Ariana Azevedo, de Menezes Rabelo, Erica, Nascimento-Neto, Luiz Gonzaga, Arruda, Francisco Vassiliepe Sousa, Vasconcelos, Mayron Alves, Carneiro, Victor Alves, Cavada, Benildo Sousa, Teixeira, Edson Holanda
Format: Article
Language:English
Subjects:
Antibiotics
Antimicrobial agents
Bacteria
Bacteriology
Biofilms
Colleges & universities
Gram-negative bacteria
Laboratories
Legal medicine
Microbial mats
Microorganisms
NMR
Nuclear magnetic resonance
Spectrum analysis
Terpenes
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Summary:This study evaluated the antimicrobial effect of 3β,6β,16β-trihydroxylup-20(29)-ene (CLF1), a triterpene isolated from Combretum leprosum Mart., in inhibiting the planktonic growth and biofilms of Gram positive bacteria Streptococcus mutans and S. mitis. The antimicrobial activity was assessed by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The antibiofilm potential was determined by quantifying total biomass and enumerating biofilm-entrapped viable bacteria. In addition, the acute toxicity of CLF1 on Artemia sp. nauplii was also determined. The results showed that CLF1 was able in inhibiting the growth of S. mutans and S. mitis with MIC and MBC of 7.8 μg/mL and 15.6 μg/mL, respectively. CLF1 was highly effective on biofilms of both bacteria. Only 7.8 μg/mL CLF1 was enough to inhibit by 97% and 90% biomass production of S. mutans and S. mitis, respectively. On the other hand, such effects were not evident on Gram negative Pseudomonas aeruginosa and Klebsiella oxytoca. The toxicity tests showed that the LC50 of CLF1 was 98.19 μg/mL. Therefore, CLF1 isolated from C. leprosum may constitute an important natural agent for the development of new therapies for caries and other infectious diseases caused by S. mutans and S. mitis.
ISSN:2314-6133
2314-6141
DOI:10.1155/2014/729358