Kunjin replicon-based simian immunodeficiency virusgagvaccines

An RNA-based, non-cytopathic replicon vector system, based on the flavivirus Kunjin, has shown considerable promise as a new vaccine delivery system. Here we describe the testing in mice of four different SIVmac239gagvaccines delivered by Kunjin replicon virus-like-particles. The four vaccines encod...

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Bibliographic Details
Published in:Vaccine 2008-06, Vol.26 (26), p.3268
Main Authors: Anraku, Itaru, Mokhonov, Vladislav V, Rattanasena, Paweena, Mokhonova, Ekaterina I, Leung, Jason, Pijlman, Gorben, Cara, Andrea, Schroder, Wayne A, Khromykh, Alexander A, Suhrbier, Andreas
Format: Article
Language:English
Subjects:
Acquired immune deficiency syndrome
AIDS
Construction
Genes
HIV
Human immunodeficiency virus
Infections
Plasmids
Proteins
RNA polymerase
Vaccines
Viruses
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Summary:An RNA-based, non-cytopathic replicon vector system, based on the flavivirus Kunjin, has shown considerable promise as a new vaccine delivery system. Here we describe the testing in mice of four different SIVmac239gagvaccines delivered by Kunjin replicon virus-like-particles. The four vaccines encoded the wild typegaggene, an RNA-optimisedgaggene, a codon-optimisedgaggene and a modifiedgag-polgene construct. The vaccines behaved quite differently for induction of effector memory and central memory responses, for mediation of protection, and with respect to insert stability, with the SIVgag-polvaccine providing the optimal performance. These results illustrate that for an RNA-based vector the RNA sequence of the antigen can have profound and unforeseen consequences on vaccine behaviour.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2008.04.001