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Vaccination with Virus-Like Particles Induces Long Lasting Protection from Experimentally Induced Sarcoid-Like Tumours in Horses
Reasons for performing study We have already demonstrated that vaccination with empty BPV1 capsids termed virus‐like particles (VLP) protects horses from experimental infection with BPV1 virion. Long‐term monitoring of antibody titres in experimental horses and data from other species suggest that t...
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Published in: | Equine veterinary journal 2014-09, Vol.46 (S47), p.15-16 |
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container_title | Equine veterinary journal |
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creator | Hainisch, E.K. Harnacker, J. Shafti-Keramat, S. Kirnbauer, R. Brandt, S. |
description | Reasons for performing study
We have already demonstrated that vaccination with empty BPV1 capsids termed virus‐like particles (VLP) protects horses from experimental infection with BPV1 virion. Long‐term monitoring of antibody titres in experimental horses and data from other species suggest that this protection is long lasting.
Objectives
To test protection against experimental infection with BPV‐1 virion in horses that were vaccinated with BPV‐1 L1 VLP approximately 5 years earlier.
Study design
Controlled experiment.
Methods
Seven horses, vaccinated 3 times (boosters after 4 weeks and 6 months) in 2007/2008 with doses of BPV1 L1 VLP ranging from 50 μg to 150 μg/dose and 3 unvaccinated control horses were challenged by intra‐dermal inoculation with cow wart derived BPV1 virion (5 × 107 BPV‐1 virions per wheal, 10 wheals per horse). Inoculation sites were monitored for 10 weeks. Blood for serum antibody titre determination by pseudovirion neutralisation assay was taken on the day of challenge and after 6 months.
Results
Six of 7 vaccinated horses had measurable serum antibody titres (1:50 to 1:400). These titres were boosted by inoculation (about one step of dilution). Two of 3 unvaccinated controls remained sero‐negative. One control horse showed sero‐conversion. All control horses developed tumours at all 10 inoculation sites. Tumours appeared approximately 2 weeks after inoculation and reached maximum sizes of up to 8 mm. Regression was complete by 8 weeks after their first appearance in all horses. All vaccinated horses remained completely free from tumours. No influence of dose rate or antibody titre on the level of protection could be determined.
Conclusions
BPV‐1 L1 VLP vaccination proves to be fully effective in protecting horses from experimental infection and tumour formation 5 years post immunisation. The protection was complete even in horses with low or unmeasurable antibody titres. This is another step towards establishing a vaccination against equine sarcoids.
Ethical animal research: This experiment was approved by the institutional ethics committee and the national authority under license No: GZ: 68.205/0144‐II/3b/2012. Sources of funding: Veterinary University Vienna, Austrian Research Fund (FWF). Competing interests: None. |
doi_str_mv | 10.1111/evj.12323_33 |
format | article |
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We have already demonstrated that vaccination with empty BPV1 capsids termed virus‐like particles (VLP) protects horses from experimental infection with BPV1 virion. Long‐term monitoring of antibody titres in experimental horses and data from other species suggest that this protection is long lasting.
Objectives
To test protection against experimental infection with BPV‐1 virion in horses that were vaccinated with BPV‐1 L1 VLP approximately 5 years earlier.
Study design
Controlled experiment.
Methods
Seven horses, vaccinated 3 times (boosters after 4 weeks and 6 months) in 2007/2008 with doses of BPV1 L1 VLP ranging from 50 μg to 150 μg/dose and 3 unvaccinated control horses were challenged by intra‐dermal inoculation with cow wart derived BPV1 virion (5 × 107 BPV‐1 virions per wheal, 10 wheals per horse). Inoculation sites were monitored for 10 weeks. Blood for serum antibody titre determination by pseudovirion neutralisation assay was taken on the day of challenge and after 6 months.
Results
Six of 7 vaccinated horses had measurable serum antibody titres (1:50 to 1:400). These titres were boosted by inoculation (about one step of dilution). Two of 3 unvaccinated controls remained sero‐negative. One control horse showed sero‐conversion. All control horses developed tumours at all 10 inoculation sites. Tumours appeared approximately 2 weeks after inoculation and reached maximum sizes of up to 8 mm. Regression was complete by 8 weeks after their first appearance in all horses. All vaccinated horses remained completely free from tumours. No influence of dose rate or antibody titre on the level of protection could be determined.
Conclusions
BPV‐1 L1 VLP vaccination proves to be fully effective in protecting horses from experimental infection and tumour formation 5 years post immunisation. The protection was complete even in horses with low or unmeasurable antibody titres. This is another step towards establishing a vaccination against equine sarcoids.
Ethical animal research: This experiment was approved by the institutional ethics committee and the national authority under license No: GZ: 68.205/0144‐II/3b/2012. Sources of funding: Veterinary University Vienna, Austrian Research Fund (FWF). Competing interests: None.</description><identifier>ISSN: 0425-1644</identifier><identifier>EISSN: 2042-3306</identifier><identifier>DOI: 10.1111/evj.12323_33</identifier><identifier>CODEN: EQVJAI</identifier><language>eng</language><publisher>Fordham: Blackwell Publishing Ltd</publisher><ispartof>Equine veterinary journal, 2014-09, Vol.46 (S47), p.15-16</ispartof><rights>2014 The Author(s). Equine Veterinary Journal © 2014 EVJ Ltd</rights><rights>2014 EVJ Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2303-8e714c0862a5e721e024b137924431b8ed89aea20c40bc8c629fe03b5bae17c73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Hainisch, E.K.</creatorcontrib><creatorcontrib>Harnacker, J.</creatorcontrib><creatorcontrib>Shafti-Keramat, S.</creatorcontrib><creatorcontrib>Kirnbauer, R.</creatorcontrib><creatorcontrib>Brandt, S.</creatorcontrib><title>Vaccination with Virus-Like Particles Induces Long Lasting Protection from Experimentally Induced Sarcoid-Like Tumours in Horses</title><title>Equine veterinary journal</title><addtitle>Equine Vet J</addtitle><description>Reasons for performing study
We have already demonstrated that vaccination with empty BPV1 capsids termed virus‐like particles (VLP) protects horses from experimental infection with BPV1 virion. Long‐term monitoring of antibody titres in experimental horses and data from other species suggest that this protection is long lasting.
Objectives
To test protection against experimental infection with BPV‐1 virion in horses that were vaccinated with BPV‐1 L1 VLP approximately 5 years earlier.
Study design
Controlled experiment.
Methods
Seven horses, vaccinated 3 times (boosters after 4 weeks and 6 months) in 2007/2008 with doses of BPV1 L1 VLP ranging from 50 μg to 150 μg/dose and 3 unvaccinated control horses were challenged by intra‐dermal inoculation with cow wart derived BPV1 virion (5 × 107 BPV‐1 virions per wheal, 10 wheals per horse). Inoculation sites were monitored for 10 weeks. Blood for serum antibody titre determination by pseudovirion neutralisation assay was taken on the day of challenge and after 6 months.
Results
Six of 7 vaccinated horses had measurable serum antibody titres (1:50 to 1:400). These titres were boosted by inoculation (about one step of dilution). Two of 3 unvaccinated controls remained sero‐negative. One control horse showed sero‐conversion. All control horses developed tumours at all 10 inoculation sites. Tumours appeared approximately 2 weeks after inoculation and reached maximum sizes of up to 8 mm. Regression was complete by 8 weeks after their first appearance in all horses. All vaccinated horses remained completely free from tumours. No influence of dose rate or antibody titre on the level of protection could be determined.
Conclusions
BPV‐1 L1 VLP vaccination proves to be fully effective in protecting horses from experimental infection and tumour formation 5 years post immunisation. The protection was complete even in horses with low or unmeasurable antibody titres. This is another step towards establishing a vaccination against equine sarcoids.
Ethical animal research: This experiment was approved by the institutional ethics committee and the national authority under license No: GZ: 68.205/0144‐II/3b/2012. Sources of funding: Veterinary University Vienna, Austrian Research Fund (FWF). Competing interests: None.</description><issn>0425-1644</issn><issn>2042-3306</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp9kM1OGzEUhS1EJQJ01wewxJYJtq_nJ0t-QqAaCmppkNhYHucGHCbjYM8UsuPR6zKAWNWb48X3XV8fQr5xNuTxHOCfxZALEKAANshAMCkSAJZtkkG8pgnPpNwi2yEsGAMQUgzIy1QbYxvdWtfQJ9ve06n1XUhK-4D0SvvWmhoDPW9mnYlZuuaOljq0NuaVdy2aV3Pu3ZKOn1fo7RKbVtf1-s2Z0V_aG2dn_cjrbuk6H6ht6JnzAcMu-TLXdcCvb7lDfp-Or4_PkvJycn58WCZGAIOkwJxLw4pM6BRzwZEJWXHIR0JK4FWBs2KkUQtmJKtMYTIxmiODKq008tzksEP2-rkr7x47DK1axEWa-KTiacZSKXgOkdrvKeNdCB7nahV_pP1acab-daxix-q944inPf5ka1z_l1Xj6fdPXtJ7NrT4_OFp_6CyHPJU3fyYqJOLUXl7O_mpjuAvKVKQMg</recordid><startdate>201409</startdate><enddate>201409</enddate><creator>Hainisch, E.K.</creator><creator>Harnacker, J.</creator><creator>Shafti-Keramat, S.</creator><creator>Kirnbauer, R.</creator><creator>Brandt, S.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope></search><sort><creationdate>201409</creationdate><title>Vaccination with Virus-Like Particles Induces Long Lasting Protection from Experimentally Induced Sarcoid-Like Tumours in Horses</title><author>Hainisch, E.K. ; Harnacker, J. ; Shafti-Keramat, S. ; Kirnbauer, R. ; Brandt, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2303-8e714c0862a5e721e024b137924431b8ed89aea20c40bc8c629fe03b5bae17c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hainisch, E.K.</creatorcontrib><creatorcontrib>Harnacker, J.</creatorcontrib><creatorcontrib>Shafti-Keramat, S.</creatorcontrib><creatorcontrib>Kirnbauer, R.</creatorcontrib><creatorcontrib>Brandt, S.</creatorcontrib><collection>Istex</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>Equine veterinary journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hainisch, E.K.</au><au>Harnacker, J.</au><au>Shafti-Keramat, S.</au><au>Kirnbauer, R.</au><au>Brandt, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vaccination with Virus-Like Particles Induces Long Lasting Protection from Experimentally Induced Sarcoid-Like Tumours in Horses</atitle><jtitle>Equine veterinary journal</jtitle><addtitle>Equine Vet J</addtitle><date>2014-09</date><risdate>2014</risdate><volume>46</volume><issue>S47</issue><spage>15</spage><epage>16</epage><pages>15-16</pages><issn>0425-1644</issn><eissn>2042-3306</eissn><coden>EQVJAI</coden><abstract>Reasons for performing study
We have already demonstrated that vaccination with empty BPV1 capsids termed virus‐like particles (VLP) protects horses from experimental infection with BPV1 virion. Long‐term monitoring of antibody titres in experimental horses and data from other species suggest that this protection is long lasting.
Objectives
To test protection against experimental infection with BPV‐1 virion in horses that were vaccinated with BPV‐1 L1 VLP approximately 5 years earlier.
Study design
Controlled experiment.
Methods
Seven horses, vaccinated 3 times (boosters after 4 weeks and 6 months) in 2007/2008 with doses of BPV1 L1 VLP ranging from 50 μg to 150 μg/dose and 3 unvaccinated control horses were challenged by intra‐dermal inoculation with cow wart derived BPV1 virion (5 × 107 BPV‐1 virions per wheal, 10 wheals per horse). Inoculation sites were monitored for 10 weeks. Blood for serum antibody titre determination by pseudovirion neutralisation assay was taken on the day of challenge and after 6 months.
Results
Six of 7 vaccinated horses had measurable serum antibody titres (1:50 to 1:400). These titres were boosted by inoculation (about one step of dilution). Two of 3 unvaccinated controls remained sero‐negative. One control horse showed sero‐conversion. All control horses developed tumours at all 10 inoculation sites. Tumours appeared approximately 2 weeks after inoculation and reached maximum sizes of up to 8 mm. Regression was complete by 8 weeks after their first appearance in all horses. All vaccinated horses remained completely free from tumours. No influence of dose rate or antibody titre on the level of protection could be determined.
Conclusions
BPV‐1 L1 VLP vaccination proves to be fully effective in protecting horses from experimental infection and tumour formation 5 years post immunisation. The protection was complete even in horses with low or unmeasurable antibody titres. This is another step towards establishing a vaccination against equine sarcoids.
Ethical animal research: This experiment was approved by the institutional ethics committee and the national authority under license No: GZ: 68.205/0144‐II/3b/2012. Sources of funding: Veterinary University Vienna, Austrian Research Fund (FWF). Competing interests: None.</abstract><cop>Fordham</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/evj.12323_33</doi><tpages>2</tpages><oa>free_for_read</oa></addata></record> |
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title | Vaccination with Virus-Like Particles Induces Long Lasting Protection from Experimentally Induced Sarcoid-Like Tumours in Horses |
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