Histopathological changes and apoptosis detection in canine myxomatous mitral valve disease using tissue microarray technique

Myxomatous mitral valve disease (MMVD) is the most common cardiac disease in dogs. The etiology of MMVD is currently unclear. The cell density has been found increasingly in myxomatous mitral valves. The proliferation is unlikely a major mechanism of increased cell density in diseased valves. Other...

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Bibliographic Details
Published in:Comparative clinical pathology 2014-09, Vol.23 (5), p.1173-1178
Main Authors: Jiranantasak, Treenate, Rungsipipat, Anudep, Surachetpong, Sirilak
Format: Article
Language:English
Subjects:
Hematology
Medicine
Medicine & Public Health
Oncology
Original Article
Pathology
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Summary:Myxomatous mitral valve disease (MMVD) is the most common cardiac disease in dogs. The etiology of MMVD is currently unclear. The cell density has been found increasingly in myxomatous mitral valves. The proliferation is unlikely a major mechanism of increased cell density in diseased valves. Other mechanisms such as anti-apoptosis or cell migration may be involved. The objective of this study was to determine the apoptotic cells within MMVD valves by assessing the immunoreactivity of cleaved caspase-3 and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) assay using tissue microarray technique. Fifteen normal and 40 MMVD valves were included in the study. TUNEL marker and cleaved caspase-3 expressions were significantly decreased in myxomatous valves compared to normal valves ( p  
ISSN:1618-5641
1618-565X
DOI:10.1007/s00580-013-1759-8