Relations between macro- and microstability of C^sub H^2 domains of human IgG2 and their biological activity: 2. Calculation of thermodynamic functions that characterize the domain stability

Thermodynamic parameters of stabilization of the native structure of C^sub H^2 domains in human myeloma second subclass LOM and SIN immunoglobulins and their hFc fragments, which we obtained for the first time, were determined using the method of scanning microcalorimetry. The decrease in thermal st...

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Bibliographic Details
Published in:Molecular biology (New York) 2014-09, Vol.48 (5), p.734
Main Author: Tishchenko, V M
Format: Article
Language:English
Subjects:
Immunoglobulins
Molecular biology
Thermodynamics
Online Access:Get full text
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Summary:Thermodynamic parameters of stabilization of the native structure of C^sub H^2 domains in human myeloma second subclass LOM and SIN immunoglobulins and their hFc fragments, which we obtained for the first time, were determined using the method of scanning microcalorimetry. The decrease in thermal stability and energy of stabilization of the native structure of the domains in intact proteins in the physiological range of temperatures (20-37°C) correlated mainly with the entropy factor.[PUBLICATION ABSTRACT]
ISSN:0026-8933
1608-3245
DOI:10.1134/S002689331405015X