Screening survey of co-production of fusaric acid, fusarin C, and fumonisins B^sub 1^, B^sub 2^ and B^sub 3^ by Fusarium strains grown in maize grains

• Published in: Mycotoxin research 2014-11, Vol.30 (4), p.231
• Main Authors: Han, Z, Tangni, E K, Huybrechts, B, Munaut, F, Scauflaire, J, Wu, A, Callebaut, A
• Format: Article
• Language: English
• Subjects: Corn; Microbiology; Mycotoxins; Toxins
• ISSN: 0178-7888; 1867-1632
• DOI: 10.1007/s12550-014-0207-1

Fusarium species isolated from Belgian maize were screened for their ability to produce fusarin C, fusaric acid, fumonisins B^sub 1^ (FB^sub 1^), FB^sub 2^ and FB^sub 3^ in maize grains. First, cultivation of Fusarium species in Myro liquid medium allowed overcoming the shortage of the standard of fusarin C on the market. All Fusarium verticillioides produced much higher contents of mycotoxins in Myro compared to Fusarium graminearum or Fusarium venenatum. The optimization of the LC-MS/MS method resulted in low limits of detection and quantification for fusarin C, fusaric acid, FB^sub 1^, FB^sub 2^ and FB^sub 3^ determination in maize grains. Its application for screening the potential toxin production ability evidenced that the concentrations of the analytes were significantly increased at various levels when F. verticillioides strains were cultivated in maize grains and reached 441 mg kg^sup -1^ for fusaric acid, 74 mg kg^sup -1^ for fusarin C, 1,301 mg kg^sup -1^ for FB^sub 1^, 367 mg kg^sup -1^ for FB^sub 2^ and 753 mg kg^sup -1^ for FB^sub 3^.[PUBLICATION ABSTRACT]