A SURVEY ON THE ANTIMICROBIAL EFFECT OF METHANOLIC EXTRACT AND ALKALOID FRACTIONS OF AERIAL PARTS OF GLAUCIUM VITELLINUM AGAINST DIFFERENT BACTERIAL STRAINS AND CANDIDA SPP WITH DISK DIFFUSION AND MIC

Extrac- tion of aerial parts was done by perculator apparatus and using methanol 80%.Different alkaloids solvents such as pe- troleum and chloroform were added based on standard methods.The antimicrobial effect of methanolic extract and alkaloid extracts were evaluated against Staphylococcus aureus...

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Bibliographic Details
Published in:Iranian journal of public health 2014-01, Vol.43 (2), p.284
Main Authors: Mehrara, Mina, Rahimifard, Nahid, Hakemi-Vala, Mojdeh, Asgarpanah, Jinus, Heidary, Mohsen, Khoshnood, Saeed
Format: Article
Language:English
Subjects:
Antimicrobial agents
Diffusion
Fractions
Methods
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Summary:Extrac- tion of aerial parts was done by perculator apparatus and using methanol 80%.Different alkaloids solvents such as pe- troleum and chloroform were added based on standard methods.The antimicrobial effect of methanolic extract and alkaloid extracts were evaluated against Staphylococcus aureus (PTCC 1431), Escherichia coli (PTCC 1399), Pseudomonas aeru- ginosa (PTCC 1430), Klebsiella pneumonia (PTCC 1053), Salmo- nella typhimurium (PTCC 1639), Candida albicans (PTCC 5027) based on CLSI 2012 protocol by disk diffusion meth- od.Further microdilution was done to determine Minimal inhibitory concentration(MIC).
ISSN:2251-6085
2251-6093