Finger millet bran supplementation alleviates obesity-induced oxidative stress, inflammation and gut microbial derangements in high-fat diet-fed mice

• Published in: British journal of nutrition 2014-11, Vol.112 (9), p.1447-1458
• Main Authors: Murtaza, Nida, Baboota, Ritesh K., Jagtap, Sneha, Singh, Dhirendra P., Khare, Pragyanshu, Sarma, Siddhartha M., Podili, Koteswaraiah, Alagesan, Subramanian, Chandra, T. S., Bhutani, K. K., Boparai, Ravneet K., Bishnoi, Mahendra, Kondepudi, Kanthi Kiran
• Format: Article
• Language: English
• Subjects: Adipose Tissue - metabolism; Animals; Bacterial Load; Biological and medical sciences; Blood Glucose - metabolism; Body weight; Diabetes; Diet; Diet, High-Fat - adverse effects; Dietary Supplements; Eleusine; Epidemiology; Feeding. Feeding behavior; Functional foods & nutraceuticals; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Inflammation - prevention & control; Intestines - microbiology; Lipids - blood; Liver - metabolism; Medical sciences; Metabolic diseases; Metabolism and Metabolic Studies; Mice; Millet; Mitochondria, Muscle - metabolism; Muscle, Skeletal - metabolism; Nutrigenomics; Nutrition research; Obesity; Obesity - genetics; Obesity - metabolism; Oils & fats; Oxidative Stress; Pancreas - metabolism; Seeds; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Weight Gain
• ISSN: 0007-1145; 1475-2662
• DOI: 10.1017/S0007114514002396

Several epidemiological studies have shown that the consumption of finger millet (FM) alleviates diabetes-related complications. In the present study, the effect of finger millet whole grain (FM-WG) and bran (FM-BR) supplementation was evaluated in high-fat diet-fed LACA mice for 12 weeks. Mice were divided into four groups: control group fed a normal diet (10 % fat as energy); a group fed a high-fat diet; a group fed the same high-fat diet supplemented with FM-BR; a group fed the same high-fat diet supplemented with FM-WG. The inclusion of FM-BR at 10 % (w/w) in a high-fat diet had more beneficial effects than that of FM-WG. FM-BR supplementation prevented body weight gain, improved lipid profile and anti-inflammatory status, alleviated oxidative stress, regulated the expression levels of several obesity-related genes, increased the abundance of beneficial gut bacteria (Lactobacillus, Bifidobacteria and Roseburia) and suppressed the abundance of Enterobacter in caecal contents (P≤ 0·05). In conclusion, FM-BR supplementation could be an effective strategy for preventing high-fat diet-induced changes and developing FM-BR-enriched functional foods.