Immunogenicity and antigenic properties of Pf332-C231, a fragment of a non-repeat region of thePlasmodium falciparumantigen Pf332

Antigen Pf332, a megadalton protein has been shown to be associated with the membrane of infected erythrocytes. Detailed functional studies on the antigen have remained hampered by the cross-reactive nature of antibodies generated to Pf332. Pf332-C231, identified in the C-terminal region of Pf332 wa...

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Bibliographic Details
Published in:Vaccine 2009-12, Vol.28 (1), p.90
Main Authors: Balogun, HA, Vasconcelos, N-M, Lindberg, R, Haeggström, M, Moll, K, Chen, Q, Wahlgren, M, Berzins, K
Format: Article
Language:English
Subjects:
Chromatography
Cloning
Immunogenicity
Malaria
Parasites
Polymerase chain reaction
Protein expression
Proteins
Studies
Vector-borne diseases
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Summary:Antigen Pf332, a megadalton protein has been shown to be associated with the membrane of infected erythrocytes. Detailed functional studies on the antigen have remained hampered by the cross-reactive nature of antibodies generated to Pf332. Pf332-C231, identified in the C-terminal region of Pf332 was cloned and antibodies against the C231 fragment were shown to react with intact Pf332 antigen by both immunofluorescence and immunoblotting analyses. Antibodies to C231 inhibitedin vitro Plasmodium falciparumgrowth efficiently. In addition, human sera from malaria-exposed individuals reacted with recombinant C231. We show that Pf332-C231 represents a functional domain and is expected to facilitate further studies on Pf332 as a potential target for protective immune responses and the function of the antigen.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2009.09.110