Prevalence and molecular characterization ofHepatozoon canisin dogs from urban and rural areas in Southeast Brazil

The objective of this survey was to investigate the prevalence ofHepatozooninfection in dogs in the rural and urban areas of Uberlândia, Brazil by PCR and molecular characterization. DNA was obtained from blood samples collected from 346 local dogs from both genders and various ages. Seventeen PCR p...

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Bibliographic Details
Published in:Research in veterinary science 2014-10, Vol.97 (2), p.325
Main Authors: de Miranda, RL, O'Dwyer, LH, de Castro, JR, Metzger, B, Rubini, AS, Mundim, AV, Eyal, O, Talmi-Frank, D, Cury, MC, Baneth, G
Format: Article
Language:English
Subjects:
Age
Arachnids
Deoxyribonucleic acid
DNA
Dogs
Gender
Infections
Rural areas
Urban areas
Veterinary medicine
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Summary:The objective of this survey was to investigate the prevalence ofHepatozooninfection in dogs in the rural and urban areas of Uberlândia, Brazil by PCR and molecular characterization. DNA was obtained from blood samples collected from 346 local dogs from both genders and various ages. Seventeen PCR products from positive blood samples of urban dogs and 13 from the rural dogs were sequenced. Partial sequences of the 18S rRNA gene indicated that all 30 dogs were infected withHepatozoon canissimilar in sequence toH. canisfrom southern Europe. Four local dog sequences were submitted to GenBank (accessions JN835188; KF692038; KF692039; KF692040). This study indicates thatH. canisis the cause of canine hepatozoonosis in Uberlândia and that infection is similarly widespread in rural and urban dogs.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2014.06.015