Effects ofElaeagnus angustifoliaL. supplementation on serum levels of inflammatory cytokines and matrix metalloproteinases in females with knee osteoarthritis

• Published in: Complementary therapies in medicine 2014-10, Vol.22 (5), p.864
• Main Authors: Nikniaz, Zeinab, Ostadrahimi, Alireza, Mahdavi, Reza, Ebrahimi, Ali asghar, Nikniaz, Leila
• Format: Article
• Language: English
• Subjects: Arthritis; Body mass index; Clinical trials; Collagen; Cytokines; Drugs; Nutrition research; Polyphenols; Statistical analysis; Studies; Variance analysis
• ISSN: 0965-2299; 1873-6963
• DOI: 10.1016/j.ctim.2014.07.004

In an attempt to investigate new strategies aimed at reducing inflammation in osteoarthritis, the anti-inflammatory effect ofElaeagnus angustifoliaL. as a complementary treatment was evaluated in females with knee osteoarthritis. Method In this clinical trial, 90 females with mild to moderate osteoarthritis were assigned to two intervention and one placebo groups. In addition to the conventional therapy, the patients in intervention groups received 15g/day ofE. angustifoliaL. medulla and whole fruit powders respectively for 8 weeks. The levels of tumor necrosis factor-alpha (TNF-), interleukine-1β (IL-1β), interleukine-10 (IL-10), matrix metalloproteinase-1 (MMP-1) and -13 (MMP-13) were measured with human ELISA kits. Pairedt-test and ANOVA were used for statistical analysis. Results The statistically significant decrease was observed in the mean levels of serum TNF- in the medulla (0.004) and whole fruit (0.001) groups after 8 weeks of supplementation. In contrast to the placebo group, there was a significant rise in the mean levels of serum IL-10 in medulla (p-value=0.01) and whole fruit groups (p-value=0.009) at the end of study. The interventions resulted in significant decrease in the mean levels of serum MMP-1 in the medulla (0.001) and whole fruit (0.002) groups. After the interventions, no significant changes were observed in the serum IL-1β and MMP-13 levels. Conclusion Daily supplementation withE. angustifoliaL. in both forms of medulla and whole fruit powders appeared to be effective for decreasing inflammatory cytokines (TNF- and MMP-1) and enhancing anti-inflammatory cytokines (IL-10).