Integrase-defective lentiviral vectors encoding cytokines induce differentiation of human dendritic cells and stimulate multivalent immune responsesin vitroandin vivo

Highlights * Integrase-defective lentiviral vectors (ID-LVs) efficiently infect DC precursors. * Integration of ID-LVs into the genome is dramatically reduced, enhancing safety. * ID-LVs co-expressing GM-CSF/IL-4 or GM-CSF/IFN-α induce monocytes to differentiate into DCs. * Lentivirus-induced DCs (i...

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Bibliographic Details
Published in:Vaccine 2012-07, Vol.30 (34), p.5118
Main Authors: Daenthanasanmak, Anusara, Salguero, Gustavo, Borchers, Sylvia, Figueiredo, Constanca, Jacobs, Roland, Sundarasetty, Bala Sai, Schneider, Andreas, Schambach, Axel, Eiz-Vesper, Britta, Blasczyk, Rainer, Weissinger, Eva M, Ganser, Arnold, Stripecke, Renata
Format: Article
Language:English
Subjects:
Antigens
Cytomegalovirus
Cytotoxicity
Design of experiments
Good Manufacturing Practice
Hematology
Immune system
Laboratories
Lymphocytes
Rodents
T cell receptors
Transplants & implants
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Summary:Highlights * Integrase-defective lentiviral vectors (ID-LVs) efficiently infect DC precursors. * Integration of ID-LVs into the genome is dramatically reduced, enhancing safety. * ID-LVs co-expressing GM-CSF/IL-4 or GM-CSF/IFN-α induce monocytes to differentiate into DCs. * Lentivirus-induced DCs (iDCs) can be co-programmed with full length antigens. * Human iDCs co-expressing a CMV antigen stimulated CD8+T cellsin vitroandin vivo.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2012.05.063