Two live attenuatedShigella flexneri2a strains WRSf2G12 and WRSf2G15: A new combination of gene deletions for 2nd generation live attenuated vaccine candidates

Highlights * New, second-generation live attenuatedShigella flexneri2a vaccine candidates. * WRSf2G12 and WRSf2G15 are as invasive as parental strain 2457T in HeLa cells. * WRSf2G12 and WRSf2G15 are Sereny negative and protective in guinea pigs. * WRSf2G15 that lacks the MsbB2 gene has lower endotox...

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Bibliographic Details
Published in:Vaccine 2012-07, Vol.30 (34), p.5159
Main Authors: Ranallo, Ryan T, Fonseka, Suramya, Boren, Tara L, Bedford, Lisa A, Kaminski, Robert W, Thakkar, Sejal, Venkatesan, Malabi M
Format: Article
Language:English
Subjects:
Antibiotics
Antigens
Bacteria
Bacteriology
E coli
Fever
Genes
Immunization
Infections
Lipids
Plasmids
Proteins
Rodents
Shigellosis
Vaccines
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Summary:Highlights * New, second-generation live attenuatedShigella flexneri2a vaccine candidates. * WRSf2G12 and WRSf2G15 are as invasive as parental strain 2457T in HeLa cells. * WRSf2G12 and WRSf2G15 are Sereny negative and protective in guinea pigs. * WRSf2G15 that lacks the MsbB2 gene has lower endotoxicity profile in macrophages. * A 3 vs. 2 dose immunization regimen provides better protection in guinea pigs.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2012.05.003