Validation of a lateral flow test (MRLAFMQ) for the detection of aflatoxin M^sub 1^ at 50 ng l^sup -1^ in raw commingled milk

Aflatoxin M1 contamination in dairy products is a risk when feedstuff contaminated with aflatoxin B1 produced by moulds is consumed by milk-producing animals. Milk can be screened for aflatoxin M1 at the European Union maximum limit of 50 ng l... by a lateral flow test, the MRLAFMQ (Aflatoxin M1) Te...

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Bibliographic Details
Published in:Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment Chemistry, analysis, control, exposure & risk assessment, 2014-12, Vol.31 (12), p.2080
Main Authors: Reybroeck, Wim, Ooghe, Sigrid, Saul, Steven J, Salter, Robert S
Format: Article
Language:English
Subjects:
Dairy products
Food contamination & poisoning
Food science
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Summary:Aflatoxin M1 contamination in dairy products is a risk when feedstuff contaminated with aflatoxin B1 produced by moulds is consumed by milk-producing animals. Milk can be screened for aflatoxin M1 at the European Union maximum limit of 50 ng l... by a lateral flow test, the MRLAFMQ (Aflatoxin M1) Test. The method takes 15 min with no milk dilution or a sample preparation step. The lateral flow assay was validated at the Technology and Food Science Unit of the Institute for Agricultural and Fisheries Research (ILVO-T&V) according to European Union guidelines using fortified raw milk samples. A detection capability of 50 ng l... was demonstrated with a false negative rate lower than 2% at 50 ng l... and a false positive rate of less than 0.3%. Quantitative readings had a mean bias of +2 to 6 ng l... at 50 ng l... with a standard deviation of 5-8 ng l... Based on the validation results, the test could be considered appropriate for milk screening prior to milk unload at dairies. (ProQuest: ... denotes formulae/symbols omitted.)
ISSN:1944-0049
1944-0057