Vaccination of pigs with attenuatedLawsonia intracellularisinduced acute phase protein responses and primed cell-mediated immunity without reduction in bacterial shedding after challenge

Background Lawsonia intracellulariscauses porcine proliferative enteropathy and is one of the most economically important diseases in modern pig production worldwide. The Enterisol®Ileitis vaccine have been shown to reduce clinical disease and to increase weight gain, however, while the natural infe...

Full description

Saved in:
Bibliographic Details
Published in:Vaccine 2015-01, Vol.33 (1), p.156
Main Authors: Riber, Ulla, Heegaard, Peter MH, Cordes, Henriette, Ståhl, Marie, Jensen, Tim Kåre, Jungersen, Gregers
Format: Article
Language:English
Subjects:
Antibiotics
Antigens
Cell growth
Deoxyribonucleic acid
DNA
Economic importance
Flow cytometry
Hogs
Immune system
Infections
Lymph nodes
Lymphatic system
Proteins
Swine production
Vaccines
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background Lawsonia intracellulariscauses porcine proliferative enteropathy and is one of the most economically important diseases in modern pig production worldwide. The Enterisol®Ileitis vaccine have been shown to reduce clinical disease and to increase weight gain, however, while the natural infection withL. intracellulariscan provide complete protection against re-infection, this has not been achieved by this vaccine. We therefore undertook a detailed characterization of immune responses toL. intracellularisinfection in vaccinated pigs (VAC) compared to previously infected pigs (RE) in order to pinpoint immunological determinants of protection. Results The VAC pigs shedL. intracellularisto the same extent as non-vaccinated pigs after challenge, however lessL. intracellularisin ileum and lymph nodes was seen post mortem. In the RE group, challenge did not lead toL. intracellularisshedding and no challenge bacteria were found post mortem. In both VAC and RE the acute phase haptoglobin response was diminished andL. intracellularisspecific IgG responses were delayed and reduced compared to non-vaccinated pigs. On the other handL. intracellularisspecific IFN-γ responses tended to develop faster in the VAC group compared to controls. Conclusion Although vaccinated and non-vaccinated pigs shedL. intracellularisat similar levels after challenge, a lower number of intestinalL. intracellulariswas observed in the vaccinated pigs at post mortem inspection. This might be due to the observed faster CMI responses upon challenge in vaccinated pigs. Complete protection against infection withoutL. intracellularisshedding, however, was only seen after a previous infection resulting in IFN-γ production predominantly by CD8+and CD4+CD8+cells. Improved protective vaccines againstL. intracellularisshould therefore target stimulation of these T cell subsets.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2014.10.084