Induction medium osmolality improves microspore embryogenesis in wheat and triticale

The objective of this study was to determine the effect of induction medium osmolality on embryogenesis and green plant production in wheat and triticale. Isolated microspores of wheat and triticale were subjected to a range of osmolality (300–500 mOsm kg⁻¹) using mannitol. In both species, the maxi...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2014-03, Vol.50 (1), p.121-126
Main Authors: Asif, Muhammad, Eudes, François, Randhawa, Harpinder, Amundsen, Eric, Spaner, Dean
Format: Article
Language:English
Subjects:
Albinism
Anthers
Apoptosis
Barley
Biomedical and Life Sciences
Cell Biology
Cell division
Corn
Crop production
Developmental Biology
Embryogenesis
Embryonic growth stage
Embryos
Flowers & plants
Genotypes
Life Sciences
Microspores
osmolality
Osmosis
Plant Breeding/Biotechnology
Plant cells
Plant Genetics and Genomics
Plant production
Plant Sciences
PLANT TISSUE CULTURE
Plants
Triticale
Wheat
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Summary:The objective of this study was to determine the effect of induction medium osmolality on embryogenesis and green plant production in wheat and triticale. Isolated microspores of wheat and triticale were subjected to a range of osmolality (300–500 mOsm kg⁻¹) using mannitol. In both species, the maximum number of embryo-like structures (ELS) and green plants were obtained at 350 mOsm kg⁻¹ when the induction medium was supplemented with 9.1 g L⁻¹ of mannitol. A sharp decline in microspore response was observed at higher osmolality. These results demonstrate the effect of osmolality on induction of ELS and production of green plants indicating that the process of microspore embryogenesis can be improved in wheat and triticale by increasing osmolality of the induction medium to 350 mOsm kg⁻¹.
ISSN:1054-5476
1475-2689
DOI:10.1007/s11627-013-9545-5