Regulatory T Cell Subtypes and TGF-[Beta]1 Gene Expression in Chronic Allograft Dysfunction

This article aims to evaluate the frequency of CD4+CD25+CD127- and CD3+CD8+CD28- regulatory T cells in chronic allograft dysfunction (CAD) and to investigate the expression of transforming growth factor-beta 1 (TGF-β1) in renal allografts. Thirty biopsy-proven CAD patients were pair-matched with 30...

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Bibliographic Details
Published in:Iranian journal of immunology 2014-09, Vol.11 (3), p.139
Main Authors: Assadiasl, Sara, Ahmadpoor, Pedram, Nafar, Mohsen, Pezeshki, Mahboob Lessan, Pourrezagholi, Fateme, Parvin, Mahmoud, Shahlaee, Abtin, Sepanjnia, Adel, Nicknam, Mohammad Hossein, Amirzargar, Aliakbar
Format: Article
Language:English
Subjects:
Antigens
Biopsy
Gene expression
Immunology
Long term
Lymphocytes
Proteins
Studies
Transcription factors
Transplants & implants
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Summary:This article aims to evaluate the frequency of CD4+CD25+CD127- and CD3+CD8+CD28- regulatory T cells in chronic allograft dysfunction (CAD) and to investigate the expression of transforming growth factor-beta 1 (TGF-β1) in renal allografts. Thirty biopsy-proven CAD patients were pair-matched with 30 stable graft function patients and a third group of healthy volunteers. Flow cytometry was performed on PBMCs to determine the frequency of CD3+CD8+CD28- and CD4+CD25+CD127- regulatory T cells in lymphocyt population. TGF-β1 gene expression was assessed by Real Time PCR. The percentage of CD3+CD8+CD28- Tregs among renal allograft recipients was higher than healthy controls since stable graft patients showed the most rates. The frequency of CD4+CD25+CD127- Tregs was lower in CAD patients than stable recipients and healthy group. TGF-β1 gene expression was greater in CAD patients compared to healthy group but there was no significant difference between gene expression of stable graft patients and healthy volunteers.
ISSN:1735-1383
1735-367X