Transcription and enzymatic analysis of beta-glucosidase VvBG1 in grape berry ripening

Although beta-glucosidase was recently demonstrated to be involved at transcriptional level in fleshy fruit development, it remains unclear whether BG possesses biochemical activity. In the present study, real-time reverse transcription polymerase chain reaction analysis showed that the mRNA express...

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Bibliographic Details
Published in:Plant growth regulation 2015-01, Vol.75 (1), p.67-73
Main Authors: Sun, Jing-hua, Dong, Yin-hang, Li, Chun-li, Shen, Yuan-yue
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
E coli
Fruits
Gas chromatography
Life Sciences
Mass spectroscopy
Original Paper
Plant Anatomy/Development
Plant Physiology
Plant Sciences
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Summary:Although beta-glucosidase was recently demonstrated to be involved at transcriptional level in fleshy fruit development, it remains unclear whether BG possesses biochemical activity. In the present study, real-time reverse transcription polymerase chain reaction analysis showed that the mRNA expression levels of β-glucosidase gene VvBG1 increased rapidly concomitant with berry red-colouring in Muscat Hamburg grapevine ( Vitis vinifera ), suggesting that the VvBG1 gene might be involved in the berry ripening. In order to explore VvBG1 biochemical activity, a 60-kD recombinant protein, VvBG1, was expressed in Escherichia   coli BL21 (DE3) and purified. The results gained from a combination of VvBG1-pulp-homogeneity-incubation test and gas chromatography-mass spectroscopy analysis showed that VvBG1-treated berry samples contained more free ABA than the control, demonstrating that VvBG1 has high glycosyl hydrolase activity that can transform the ABA-glucosyl ester to free ABA. In conclusion, both the high expression and increased hydrolase activity of VvBG1 could suggest an important role of ABA in grape berry ripening.
ISSN:0167-6903
1573-5087
DOI:10.1007/s10725-014-9932-x