Kinetics of o-diphenol oxidase Immobilized on Agar- Abelmoschus esculentus Polymer

DOPA is a precursor of dopamine and acts as a neuro transmitter. Oxidation of it to dopaquinone is a fast reaction by o-diphenol oxidase. Discrepancies in this reaction are responsible for diverse type of disease and disorders. The o-diphenol oxidase was purified on natural column and immobilized on...

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Bibliographic Details
Published in:Asian Journal of Research in Chemistry 2014-11, Vol.7 (11), p.2
Main Author: Giri, Anita S Goswami
Format: Article
Language:English
Online Access:Get full text
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Summary:DOPA is a precursor of dopamine and acts as a neuro transmitter. Oxidation of it to dopaquinone is a fast reaction by o-diphenol oxidase. Discrepancies in this reaction are responsible for diverse type of disease and disorders. The o-diphenol oxidase was purified on natural column and immobilized on agar-Abelmoschus esculentus polymer. Its immobilized o-diphenol oxidase activity showed Vmax = 167μmol/lit/min, Km = 2X10^sup -3^, Optimum P^sup H^ = 6.8, Optimum temperature 80 degrees Celsius, Ea = 160.45Kcal/mol and observed highest activity at 45 minutes behavior of encapsulated activity was compared with free o-diphenol oxidase.
ISSN:0974-4169
0974-4150